U.S. patents available from 1976 to present.
U.S. patent applications available from 2005 to present.

Analog circuit for an autofocus microscope system

Patent RE40674 Issued on March 24, 2009.

Patent References

Automatic focusing apparatus using scanning line weighting of a video signal to determine an in-focus condition
Patent #: 5357280
Issued on: 10/18/1994
Inventor: Yajima, et al.

Method and apparatus for checking automated optical system performance repeatability
Patent #: 5499097
Issued on: 03/12/1996
Inventor: Ortyn, et al.

Operator independent image cytometer
Patent #: 5548661
Issued on: 08/20/1996
Inventor: Price, et al.

Noise elimination circuit in negative image pickup apparatus
Patent #: 5745172
Issued on: 04/28/1998
Inventor: Miyashita

Autofocus system for scanning microscopy
Patent #: 5790710
Issued on: 08/04/1998
Inventor: Price, et al.

Optical probe microscope having a fiber optic tip that receives both a dither motion and a scanning motion, for nondestructive metrology of large sample surfaces
Patent #: 5811796
Issued on: 09/22/1998
Inventor: Marchman, et al.

Automatic focusing apparatus using scanning line weighting of a video signal to determine an in-focus condition
Patent #: 5929907
Issued on: 07/27/1999
Inventor: Yajima, et al.

Autofocus system for scanning microscopy having a volume image formation Patent #: 5932872
Issued on: 08/03/1999
Inventor: Price

Inventors

Assignee

Application

No. 10000943 filed on 11/30/2001

US Classes:

348/345Focus control

Examiners

Primary: Ho, Tuan V

Attorney, Agent or Firm

Foreign Patent References

  • WO 96/10196 WO 04/01/1996

International Class

H04N 5/202

Abstract

An analog circuit for an autofocus microscope system measures a degree of focus of an object directly from the video signal of a microscope CCD camera. The circuit then returns an index to a host computer for the purpose of adjusting the position of the microscope's objective lens to bring the object in focus. Best focus is found by comparing indices at several different vertical positions. The criterion adopted for determining the degree of focus is derived from the energy distribution of the video signal spectrum. The high frequency energy of the video spectrum is a maximum at best focus and as the optics defocus, the distribution shifts to lower frequencies. Low cost, real time autofocus is achieved with the analog circuitry of this invention, replacing more expensive dedicated, real time image processing hardware.

Other References

  • “Comparison of Phase-COntrast and Fluorescence Digital Autofocus For Scanning Microscopy”, Jeffrey H. Price & David A. Gough, Cytometry 16:283-297 (1994).
  • PCT—International Search Report (Form PCT/ISA/210).
  • PCT—Notification of Transmittal of the International Search Report (Form PCT/ISA/220) International Appl. No. PCT/US/98/00318.
  • E. Johnson et al., “Metaphase Spread Detection and Focus Using Closed Circuit Television”, The J. of Histochemistry and Cytochemistry, 22:7, pp. 536-545, XP002064321, 1974.
  • L.F. McKeogh, J.P. Sharpe & K.M. Johnson, “Design Note—A low-cost automatic translation and autofocusing system for a microscope”, Meas. Sci. Technol., No. 6, pp. 583-587, 1995.
  • Olivia et al., “Filtering out contrast reversals for microscopy autfocus”, Applied Optics vol. 38, No. 4, pp. 638-646, Feb. 1, 1999.
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