Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays

Patent 8084734 Issued on December 27, 2011. Estimated Expiration Date:

February 14, 2027. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

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Inventors

Assignee

The George Washington University

Application

No. 11674671 filed on 02/14/2007

US Classes:

250/288With sample supply means

Examiners

Primary: Berman, Jack

Attorney, Agent or Firm

Blank Rome LLP

Foreign Patent References

WO 98/20020 WO 05/01/1998
WO 98/20166 WO 05/01/1998
WO 99/57318 WO 11/01/1999
WO 00/54309 WO 09/01/2000
WO 01/50499 WO 07/01/2001
WO 01/71326 WO 09/01/2001
WO 02/093170 WO 11/01/2002
WO 02/095362 WO 11/01/2002
WO 03/025973 WO 03/01/2003
WO 2003/054543 WO 03/01/2003
WO 03/040700 WO 05/01/2003
WO 2003/079402 WO 05/01/2003
WO 2003/092581 WO 05/01/2003
WO 03/052135 WO 06/01/2003
WO 2004/064972 WO 04/01/2004
WO 2004/076511 WO 04/01/2004
WO 2004/099068 WO 05/01/2004
WO 2005/001423 WO 01/01/2005
WO 2005/017487 WO 01/01/2005
WO 2005/022583 WO 02/01/2005
WO 2005/029003 WO 02/01/2005
WO 2005/070089 WO 03/01/2005
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WO 2005/088293 WO 04/01/2005
WO 2006/083151 WO 08/01/2006
WO 2006/113785 WO 10/01/2006

International Classes

H01J 49/04
H01J 49/16

Abstract

The present invention provides a method of producing a laser-patterned silicon surface, especially silicon wafers for use in laser desorption ionization (LDI-MS) (including MALDI-MS and SELDI-MS), devices containing the same, and methods of testing samples employing the same. The surface is prepared by subjecting a silicon substrate to multiple laser shots from a high-power picosecond or femtosecond laser while in a processing environment, e.g., underwater, and generates a remarkable homogenous microcolumn array capable of providing an improved substrate for LDI-MS.

Other References

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