Immobilization of cells in a matrix formed by biocompatible charged polymers under laminar flow conditions
Patent 7700333 Issued on April 20, 2010. Estimated Expiration Date: July 26, 2024. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
This invention relates to a method for immobilizing cells under laminar flow conditions in a matrix formed by biocompatible charged polymers, to a flow device for the use with the method of the present invention, and to uses of the polymer matrices containing the cells immobilized using the method of the present invention.
Other References
Wei tan, M.S. et al. “Microfluidic Patterning of Cells in Extracellular Matrix Biopolymers: Effects of Channel Size, Cell Type, and Matrix Composition on Pattern Intergrity” Tissue Engineering, 2003, vol. 9 (2):255-267.
Toh, YC et al. “A configurable Three-Dimensional Microenvironment in a Microfluidic Channel for Primary Hepatocyte Culture” Assay and Drug Development Tehcnologies, Apr. 2005, vol. 3, No. 2: 169-176 (abstract only).
Wallace et al., “Collagen gel systems for sustained delivery and tissue engineering”, Advanced Drug Delivery Reviews, 2003, vol. 55, 1631-49.
Chia et al., “Hepatocyte encapsulation for enhanced cellular functions”, Tissue Engineering, 2000, vol. 6(5), 481-95.
Wen et al., “Microcapsules through polymer complexation Part 3: encapsulation and culture of human Burkitt lymphoma cells in vitro”, Biomaterials, 1995, vol. 16, 325-35.
Wen et al., “Microcapsules through polymer complexation I. Complex coacervation of polymers containing a high charge density”, Biomaterials, 1991, vol. 12, 374-84.
McDonald et al., “Fabrication of microfluidic systems in poly(dimethylsiloxane)”, Electrophoresis, 2000, vol. 21(1), 27-40.
Takayama et al., “Patterning cells and their environments using multiple laminar fluid flows in capillary networks”, Proc. Natl. Aca. Sci, 1999, vol. 96, 5545-48.
Schumm et al., “Enhanced viability and neuronal differentiation of neural progenitors by chromaffin cell co-culture”, Dev. Brain Res. 2002, vol. 137(2), 115-25.
Fair et al., “Induction of hepatic differentiation in embryonic stem cells by co-culture with embryonic cardiac mesoderm”, Surgery, 2003, vol. 134(2), 189-196.
Cooper et al., “C2C12 co-culture on a fibroblast substratum enables sustained survival of contractile, highly differentiated myotubes with peripheral nuclei and adult fast myosin expression”, Cell Motil. Cytoskeleton, 2004, vol. 58(3), 200-11.
Harimoto et al., “Novel approach for achieving double-layered cell sheets co-culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature-responsive culture dishes”, J. Biomed. Mater. Res., 2002, vol. 62(3), 464-70.
Liu et al., “Hybrid bio/artificial microdevices”, Biomedical Microdevices, 2002, vol. 4(4), 257-66.
Tan et al., “Microfluidic patterning of cells in extracellular matrix biopolymers: effects of channel size, cell type, and matrix composition on pattern integrity”, Tissue Engineering, 2003, vol. 9(2), 255-67.
Tan et al., “Layer-by-layer microfluidics for biomimetic three-dimensional structures”, Biomaterials, 2004, vol. 25, 1335-64.
Tan et al., (Tissue Engineering. 2003;9(2):255-267) (cited in Applicant's IDS of Oct. 28, 2004).
Alaris Medical Systems IVAC P6000 Directions for Use. 6001FAOPT73 Issue 6. Downloaded from Internet Apr. 2007.