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US Patent 7561973 - Methods for determining properties that affect an expression property value of polynucleotides in an expression system

US Patent Issued on July 14, 2009
Estimated Patent Expiration Date: Icon_subject July 31, 2028Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
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Abstract

A method of determining a property that affects expression of polynucleotides in an expression system. A plurality of polynucleotides, each encoding a polypeptide sequence, is constructed. An amino acid is encoded a plurality of times in both a first and second polynucleotide in the plurality. The amino acid is encodable by a plurality of synonymous codons including a first codon. The first codon is in the first polynucleotide at a first frequency relative to other synonymous codons, and is in the second polynucleotide at a second frequency relative to other synonymous codons. The first and second frequencies are different. Each polynucleotide is individually expressed in the expression system to measure an expression property value of the polynucleotides, thereby determining a property that affects expression of polynucleotides. The property is an effect that a frequency of use of one or more codons has on expression of polynucleotides in the expression system.

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Inventors

Assignee

Application

No. 12184240 filed on 07/31/2008

US Classes:

702/19Biological or biochemical

Examiners

Primary: Zhou, Shubo (Joe)

Attorney, Agent or Firm

US Patent References

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Foreign Patent References

  • WO 2005/013090 WO 02/01/2005
  • WO 2008/075911 WO 06/01/2008

International Classes

G01N 33/48
C12Q 1/00
C12Q 1/48
C12Q 1/68
C07H 21/02
C07H 21/04
C12Q 1/08

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