Patent 7504491 Issued on March 17, 2009. Estimated Expiration Date: June 10, 2023. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
An object of the present invention is to provide a chromoprotein derived from Cnidopus japonicus. The present invention provides a chromoprotein derived from Cnidopus japonicus having the following properties: (1) the absorption maximum wavelength is 610 nm, and fluorescence is not emitted; (2) the molar absorption coefficient is 66,700 at 610 nm; and (3) the pH sensitivity of light-absorbing property is stable at between pH 4 and pH 10.
Ngo et al. in “The Protein Folding Problem and Tertiary Structure Prediction,” 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Lukyanov et al., US 6969597, Nov. 2005, SEQ ID No. 1 sequence alignment Result 1, database: Issued—Patents—NA.
Lukyanov et al., US 6969597, Nov. 2005, SEQ ID No. 2 sequence alignment Result 1, database: Issued—Patents—NA.
Chan et al., 2006, Journal of Biological Chemistry, vol. 281, No. 49, pp. 37813-37819.
R.Y. Tsien, Ann. Rev. Biochem., vol. 67, 1998, pp. 509-544.
A. Miyawaki et al., Midoriishi, Mar. 2002, No. 13, pp. 1-4.
U.S. Appl. No. 10/516,314 to Miyawaki et al., which was filed on Dec. 10, 2004.
U.S. Appl. No. 10/525,365 to Miyawaki et al., which was filed on Feb. 23, 2005.
Hideaki Mizuno et al., “Red Fluorescent Protein from Discocoma as a Fusion Tag and a Partner for Fluorescence Resonance Energy Transfer”, Biochemistry, American Chemical society, vol. 40, No. 8, pp. 2502-2510, 2001.
Y. A. Labas et al., “Diversity and evolution of the green fluorescent protein family”, Proceedings of the National Academy of Science of USA, vol. 99, No. 7 pp. 4256-4261, 2002.
U.S. Appl. No. 10/516,214 to Miyawaki et al., filed Dec. 10, 2004.