Method for preparing para-hydroxystyrene by biocatalytic decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium
Patent 7378261 Issued on May 27, 2008. Estimated Expiration Date: 
April 14, 2024. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
April 14, 2024. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesProcess for the production of para-vinyl phenol by dehydrogenation of para-ethyl phenol Process for preparing vinylphenol polymers & copolymers Ferulic acid decarboxylase Carbon nanotube molecular labels Patent #: 6821730 InventorsAssigneeApplicationNo. 10824581 filed on 04/14/2004US Classes:435/156, Aromatic435/232, Lyase (4. )435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/410, PLANT CELL OR CELL LINE, PER SE (E.G., TRANSGENIC, MUTANT, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING, OR PRESERVING PLANT CELL OR CELL LINE; PROCESS OF ISOLATING OR SEPARATING A PLANT CELL OR CELL LINE; PROCESS OF REGENERATING PLANT CELLS INTO TISSUE, PLANT PART, OR PLANT, PER SE, WHERE NO GENOTYPIC CHANGE OCCURS; MEDIUM THEREFORE435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/252.32, Brevibacterium or corynebacterium435/252.33, Escherichia (e.g., E. coli, etc.)435/252.34, Pseudomonas435/252.35, Streptomyces435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE435/174CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOFExaminersPrimary: Ramierz, Delia M.Foreign Patent References
International ClassesC12P 7/22C12N 9/88 C12N 5/10 C12N 1/21 C12N 15/00 C12N 11/00 C12P 21/06 AbstractA biocatalytic method for preparing para-hydroxystyrene from para-hydroxycinnamic acid is described. The method uses an enzyme source having para-hydroxycinnamic acid decarboxylase activity to catalyze the decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium to produce para-hydroxystyrene, which is extracted into the organic phase of the biphasic reaction medium. The method results in a high yield of para-hydroxystyrene due to the decreased exposure of the enzyme source to the inhibitory product. The product is readily recovered from the extractant, or may be chemically derivatized directly in the extractant before recovery.Other References
Field of SearchAromaticLyase (4. ) Recombinant DNA technique included in method of making a protein or polypeptide PLANT CELL OR CELL LINE, PER SE (E.G., TRANSGENIC, MUTANT, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING, OR PRESERVING PLANT CELL OR CELL LINE; PROCESS OF ISOLATING OR SEPARATING A PLANT CELL OR CELL LINE; PROCESS OF REGENERATING PLANT CELLS INTO TISSUE, PLANT PART, OR PLANT, PER SE, WHERE NO GENOTYPIC CHANGE OCCURS; MEDIUM THEREFORE Involving tomato cell or tissue Involving corn cell or tissue Involving tobacco cell or tissue Involving soybean cell or tissue Involving cotton cell or tissue Involving sunflower cell or tissue Involving potato cell or tissue Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.) Bacillus (e.g., B. subtilis, B. thuringiensis, etc.) Brevibacterium or corynebacterium Transformants Yeast; media therefor Transformants VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) Escherichia (e.g., E. coli, etc.) CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF Pseudomonas Streptomyces ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE |
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