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Production of gamma linolenic acid by a Δ6-desaturase

Patent 7189894 Issued on March 13, 2007. Estimated Expiration Date: Icon_subject December 21, 2021. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventor

Assignee

Application

No. 10029756 filed on 12/21/2001

US Classes:

800/298, Higher plant, seedling, plant seed, or plant part (i.e., angiosperms or gymnosperms)800/281, The polynucleotide alters fat, fatty oil, ester-type wax, or fatty acid production in the plant435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/419Plant cell or cell line, per se, contains exogenous or foreign nucleic acid

Examiners

Primary: McElwain, Elizabeth F.

Attorney, Agent or Firm

Foreign Patent References

  • WO 99/27111 WO 06/01/1999

International Classes

A01H 5/00
C12N 15/82

Abstract

Linoleic acid is converted into γ-linolenic acid by the enzyme Δ6-desaturase. The present invention is directed to isolated nucleic acids comprising the Δ6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the Δ6-desaturase gene. The present invention provides recombinant constructions comprising the Δ6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.

Other References

  • Broun et al, Science 282: 131-133, Nov. 13, 1998.
  • Van de Loo et al, Proc Natl. Acad Sci, USA, 92: 6743-6747, Jul. 1995.
  • Doerks et al, TIG 14(6): 248-250, Jun. 1998.
  • Smith et al, Nature Biotechnology 15: 1222-1223, Nov. 1997.
  • Brenner, S. E., TIG 15(4): 132-133, Apr. 1999.
  • Bork et al, TIG 12(10): 425-427, Oct. 1996.
  • De Luca, V, AgBiotech News and Information 5(6): 225N-229N, 1993.
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