Methods for purifying and isolating recombinant chondroitinases

Patent 7129335 Issued on October 31, 2006. Estimated Expiration Date:

October 14, 2024. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

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Purification of heparinase I, II, and III from Flavobacterium heparinum
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Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
Patent #: 5496718
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Inventors

Application

No. 10966671 filed on 10/14/2004

US Classes:

530/412, Separation or purification435/232, Lyase (4. )536/23.2, Encodes an enzyme435/200Acting on glycosyl compound (3.2)

Examiners

Primary: Saidha, Tekchand

Attorney, Agent or Firm

Wolf, Greenfield & Sacks, P.C.

Foreign Patent References

0355831 EP 02/01/1990
0 613 949 EP 09/01/1994
1 607 478 EP 12/01/2005
54107586 JP 08/01/1979
2000044601 JP 02/01/2000
WO 91/16070 WO 10/01/1991
WO 93/08289 WO 04/01/1993
WO 94/12618 WO 06/01/1994
WO 95/13830 WO 05/01/1995
WO 95/34635 WO 12/01/1995
WO 96/01648 WO 01/01/1996
WO 96/01894 WO 01/01/1996
WO 97/16556 WO 05/01/1997
WO 97/18835 WO 05/01/1997
WO 99/58650 WO 11/01/1999
WO 200139795 WO 02/01/2000
WO 01/34781 WO 05/01/2001
WO 01/35977 WO 05/01/2001
WO 01/38399 WO 05/01/2001
WO 01/53474 WO 07/01/2001
WO 03/074080 WO 09/01/2003
WO 2004/110360 WO 12/01/2004
WO 2005/112986 WO 12/01/2005

International Classes

A23J 1/00
C12N 9/88
C07H 21/04

Abstract

The invention relates to rationally designed polysaccharide lyases and uses thereof. In particular, the invention relates to modified chondroitinase B. The modified chondroitinase B enzymes of the invention are useful for a variety of purposes, including cleaving and sequencing polysaccharides such as glycosaminoglycans (GAGs) as well as removing polysaccharides from a solution. The invention also includes methods of inhibiting anticoagulant activity, inhibiting angiogenesis, treating cancer, and inhibiting maternal malarial infection.

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