Amplification of nucleic acids with electronic detection
Patent 7090804 Issued on August 15, 2006. Estimated Expiration Date: January 27, 2019. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
422/68.1, Means for analyzing liquid or solid sample435/6, Involving nucleic acid435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/5, Involving virus or bacteriophage435/7.5, Involving avidin-biotin binding435/25, Involving oxidoreductase536/25.32, Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.)435/188, Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate422/82.01, Measuring electrical property536/26.13, Adenine or substituted adenine536/24.32, Probes for detection of microbial nucleotide sequences536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)435/91.21, Involving the making of multiple RNA copies536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA436/94, Saccharide (e.g., DNA, etc.)436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS435/91.52, Involving a ligase (6.)534/11, Actinide series metal (At. No. 89+)427/487, Polymerization of coating utilizing direct application of electrical, magnetic, wave, or particulate energy (i.e., including cross-linking, curing, and hardening of organics)422/50, ANALYZER, STRUCTURED INDICATOR, OR MANIPULATIVE LABORATORY DEVICE530/300PEPTIDES OF 3 TO 100 AMINO ACID RESIDUES
The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the nucleic acid, either directly or indirectly, to allow electronic detection of the ETM using an electrode.
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