Method for detecting protein
Patent 7067270 Issued on June 27, 2006. Estimated Expiration Date: 
December 13, 2024. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
December 13, 2024. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays Digoxigenin immunogens, antibodies, labeled conjugates, and related derivatives Digoxigenin derivatives and use thereof Patent #: 5198537 InventorApplicationNo. 11008954 filed on 12/13/2004US Classes:435/7.95, Indirect assay436/546, Fluorescent label436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/530, Cellulose or derivative436/531, Carrier is synthetic resin436/524, Carrier is inorganic436/544, Producing labeled antigens530/388.9, Binds drug, hapten, hapten--carrier complex, or specifically-identified chemical structure (e.g., theophylline, digoxin, etc.)435/7.23Tumor cell or cancer cellExaminersPrimary: Le, Long V.Assistant: Haq, Shafiqul Attorney, Agent or FirmForeign Patent References
International ClassesG01N 33/533G01N 33/532 G01N 33/535 G01N 33/543 G01N 33/545 AbstractAn object of the present invention is to provide a method for detecting a protein using a nonradioactive label which is prevented to a smaller extent than in the case of the interaction of biotin/(strepto)avidin, and which achieves higher detection sensitivity than that of the detection system using DIG. The present invention provides a method for detecting a target substance, which comprises steps of: (A) allowing a target substance to come into contact with a protein labeled with a compound having a 6-membered ring, so as to carry out a binding reaction; and (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody against the above compound having a 6-membered ring.Other References
Field of SearchInvolving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assayAssay in which a label present is an enzyme substrate or substrate analogue Indirect assay Producing labeled antigens INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS Fluorescent label Cellulose or derivative Carrier is synthetic resin Carrier is inorganic Binds drug, hapten, hapten--carrier complex, or specifically-identified chemical structure (e.g., theophylline, digoxin, etc.) Having -C(=X)-, wherein X is chalcogen attached directly or indirectly to the purine ring system by nonionic bonding or halogen bonded directly at 8-position (e.g., theophylline acetate, 8-chlorotheophylline, etc.) Caffeine per se, theobromine per se, or salt thereof |
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