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Application of biosensors for diagnosis and treatment of disease

Patent 6974706 Issued on December 13, 2005. Estimated Expiration Date: Icon_subject January 16, 2023. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

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Inventors

Assignee

Application

No. 10345532 filed on 01/16/2003

US Classes:

436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS422/84, Breath tester435/6, Involving nucleic acid435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/7.9, Assay in which an enzyme present is a label435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)436/164, OPTICAL RESULT436/172, With fluorescence or luminescence436/524, Carrier is inorganic436/528, Carrier is organic436/805, OPTICAL PROPERTY436/811, TEST FOR NAMED DISEASE, BODY CONDITION OR ORGAN FUNCTION436/815, TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDS436/900, BREATH TESTING422/91, With photometric detector435/25, Involving oxidoreductase436/151, By means of a solid body in contact with a fluid600/532, Qualitative or quantitative analysis of breath component424/9.2Testing efficacy or toxicity of a compound or composition (e.g., drug, vaccine, etc.)

Examiners

Primary: Chin, Christopher L.

Attorney, Agent or Firm

Foreign Patent References

  • WO 98/57145 WO 12/01/1998

International Class

G01N033/543

Abstract

A method for detecting compounds of interest in bodily fluids, including exhaled breath and blood. The present invention uses biosensors that mimic naturally occurring cellular mechanisms, including RNA oligonucleotide chains or “aptamers,” in combination with molecular beacons or nanotechnology to provide an effective and efficient method for diagnosing a condition and/or disease within a patient. The subject invention also provides a method for screening those analytes/biomarkers likely to be present in exhaled breath.

Other References

  • Brody et al., “The Use of Aptamers in Large Arrays for Molecular Diagnostics”, Molecular Diagnosis (1999), 4(4): 381-388.
  • Liebich and Wöll, “Volatile Substances in Blood Serum: profile Analysis and Quantitative Determination”, Journal of Chromatography (1977), 142: 505-516.
  • Paviou and Turner, “Sniffing out the Truth: Clinical Diagnosis Using the Electronic Nose”, Clin. Chem. Lab. Med. (2000), 38(2): 99-112.
  • Brody and Gold, “Aptamers as Therapeutic and Diagnostic Agents”, Reviews in Molecular Biotechnology (2000), 74: 5-13.
  • Stojanovic et al., “Aptamer-Based Folding Fluorescent Sensor for Cocaine”, J. Am. Chem. Soc. (2001), 123: 4928-4931.
  • Fischer et al., “A Man-Portable Chemical Sniffer Utilizing Novel Fluorescent Polymers for Detection of Ultra-Trace Concentrations of Explosives Emanating from Landmines”, Nomadics Inc. (2000), 1-10.
  • Rogers et al., “Fiber-Optic Biosensors Based on Total Internal-Reflection Fluorescence”, American Chemical Society (1992), Ch. 13: 165-173.
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