U.S. patents available from 1976 to present.
U.S. patent applications available from 2005 to present.

Optimized messenger RNA

Patent 6924365 Issued on August 2, 2005. Estimated Expiration Date: Icon_subject September 28, 2019. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
Patent #: 4965199
Issued on: 10/23/1990
Inventor: Capon, et al.

Overexpression of mammalian and viral proteins
Patent #: 5786464
Issued on: 07/28/1998
Inventor: Seed

High level expression of proteins
Patent #: 5795737
Issued on: 08/18/1998
Inventor: Seed, et al.

IL-2 gene expression and delivery systems and uses Patent #: 6034072
Issued on: 03/07/2000
Inventor: Ralston, et al.

Inventors

Application

No. 09407605 filed on 09/28/1999

US Classes:

536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE530/350, PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES530/381, Blood coagulation factors and fibrin, e.g., thromboplastin, etc.435/69.6, Blood proteins536/23.5, Encodes an animal polypeptide514/44Polynucleotide (e.g., RNA, DNA, etc.)

Examiners

Primary: Weber, Jon P.
Assistant: Kam, Chih-Min

Attorney, Agent or Firm

Foreign Patent References

  • 96/09378 WO 03/01/1996
  • WO 96/09378 WO 03/01/1996
  • WO 97/11086 WO 03/01/1997
  • WO 97/26333 WO 07/01/1997
  • WO 97/31115 WO 08/01/1997
  • WO 97/47358 WO 12/01/1997
  • WO 97/48370 WO 12/01/1997
  • WO 98/12207 WO 03/01/1998
  • 98/12207 WO 03/01/1998
  • WO 98/24922 WO 06/01/1998

International Classes

C07H021/04
C12N015/00

Abstract

The present invention is directed to a synthetic nucleic acid sequence which encodes a protein wherein at least one non-common codon or less-common codon is replaced by a common codon. The synthetic nucleic acid sequence can include a continuous stretch of at least 90 codons all of which are common codons.

Other References

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  • Carmel M. Lynch et al, “Sequences in the Coding Region of Clotting Factor VIII Act as Dominant Inhibitors of RNA Accumulation and Protein Production”, Human Gene Therapy, vol. 4, No. 3, pp. 259-272 (Jun. 1, 1993).
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  • Parker et al., “Translation and a 42-nucleotide segment within the coding region of the mRNA encoded by the MATα1 gene are involved in promoting rapid mRNA decay in yeast”, Proc. Natl. Acad. Sci. vol. 87, No. 7, pp 2780-2784 (1990).
  • Solomovici et al., “Does Escherichia coli Optimize the Economics of the Translation Process?”; J. theor. Biol., 185, pp 511-521 (1997).
  • Wright, “The ‘Effective number of codons’ used in a gene”, Gene, vol. 87, No. 1, pp 23-29 (1990).
  • Yang et al., “Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein”, Nucleic Acids Research, vol. 24, No. 22, pp 4592-4593 (1996).
  • Zhang et al., “An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells”, Biochemical and Biophysical Research Communications, vol. 227, No. 3, pp 707-711 (1996).
  • Zhang et al., “Graphic Analysis of Codon Usage Strategy in 1490 Human Proteins”, Journal of Protein Chemistry, vol. 12, No. 3, pp 329-335 (1993).
  • Andre et al., “Increased Immune Response Elicited by DNA Vaccination with a Synthetic gp120 Sequence with Optimized Codon Usage”; Journal of Virology, vol. 72, No. 2, pp 1497-1503 (1998).
  • Berg et al., “Growth Rate-optimized tRNA Abundance and Codon Usage”; J. Mol. Biol., pp 544-550 (1997).
  • Billinton, et al., “Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor”; Biosensors& Bioelectronics 13 pp 831-838 (1998).
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