Process for continuous purification and concentration of leukocytes from blood
Patent 6743624 Issued on June 1, 2004. Estimated Expiration Date: 
March 23, 2019. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
March 23, 2019. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesExtracts of the hemopoietic system Method for separating oil and water emulsions Method for creating a leukocyte rich sample from a mixed population of blood cells Patent #: 5785869 InventorApplicationNo. 09646985 filed on 03/23/1999US Classes:435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE435/2, MAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT435/283.1, APPARATUS435/306.1, Involving lysis of a microorganism by means other than comminution435/372Blood, lymphatic, or bone marrow origin or derivativeExaminersPrimary: Saunders, DavidAttorney, Agent or FirmInternational ClassC12N 500AbstractProcess and apparatus for the continuous purification and concentration of leukocytes from blood, characterized in that said process comprises the following steps: (a) seperating plasma from the blood by filtration in order to achieve a filtered buffy coat fraction; (b) adding an aqueous solution, which is hypotonic in relation to plasma, to the buffy coat fraction from step (a), in order to achieve lysation of erythrocytes contained in the buffy coat fraction; (c) mixing the buffy coat fraction and the aqueous hypotonic solution from step (b) in a mixing device; (d) leading the mixture from step (c) through a retention vessel; (e) leading the mixture from step (d) through a centrifuge in order to seperate the leukocytes; (f) collecting the separated leukocytes from step (e).Other References
Field of SearchMAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENTANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE Blood, lymphatic, or bone marrow origin or derivative APPARATUS Involving lysis of a microorganism by means other than comminution Producing interferons |
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