Electronic device for performing active biological operations and method of using same
Patent 6726880 Issued on April 27, 2004. Estimated Expiration Date: June 19, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
422/68.1, Means for analyzing liquid or solid sample422/50, ANALYZER, STRUCTURED INDICATOR, OR MANIPULATIVE LABORATORY DEVICE422/129, CHEMICAL REACTOR435/6, Involving nucleic acid435/7.1Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay
An electronic device for performing biological operations includes a support substrate and an array of microlocations disposed on the substrate. The array of microlocations include electronically addressable electrodes. A first collection electrode is disposed on the substrate and adjacent to a first side of the array of microlocations. A second collection electrode is disposed on the substrate and adjacent to a second side of the array of microlocations, the second side of the array being opposite of the first side such that the array of microlocations is disposed between the first and second collection electrodes. A flow cell is supported on the substrate. Preferably, the combined area of the collection electrodes is a substantial fraction, preferably at least 50% of the area of the footprint of the flow cell.
Abrams et al. “Comprehensive Detection of Single Base Changes In Human Genomic DNA Using Denaturing Gradient Gel Electrophoresis & a GC Clamp”. Genomics, 7, 1990, 463-475.
Anand and Southern “Pulsed Field Gel Electrophoresis,” Gel Electrophoresis of Nucleic Acids—A Practical Approach, 2d. Ed., D. Rickwood and B.D. Hames (New York:IRL Press 1990), pp 101-123.
Anderson and Young, “Quantitative Filter Hybridization,” Nucleic Acid Hybridization—A Practical Approach, Eds. B.D. Hames and S.J. Higgins (Washington, D.C. :IRL Press 1985) pp 73-111.
Bains, “Setting a Sequence to Sequence a Sequence,” Bio/Technology, 10:757-758 (1992).