High throughput method and system for screening candidate compounds for activity against target ion channels
Patent 6686193 Issued on February 3, 2004. Estimated Expiration Date: March 12, 2021. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/285.2, With means for applying an electric current or charge (e.g., electrofusion, electroporation, etc.)435/287.1, Including measuring or testing435/288.4, Including multiple compartments (e.g., wells, etc.)435/288.7Including optical measuring or testing means
Drug candidate screening methods are applied to discover compounds with activity against ion channel targets. The method may include modulating the transmembrane potential of host cells in a plurality of sample wells with a repetitive application of electric fields so as to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.
Other References
Gonzalez, J.E., et al., "Cell-based assays and instrumentation for screening ion-channel targets", Drug Discover Today, vol. 4, No. 9, pp. 431-439, (Sep. 1999)
Gonzalez, J.E., et al., "Voltage sensing by fluorescence resonance energy transfer in single cells", Biophysical Journal, vol. 69, No. 4, pp. 1272-1280, (Oct. 1995)
Giuliano, K.A., et al., "Fluorescent-protein biosensors: new tools for drug discovery, Trends in Biotechnology", vol. 16, No. 3, pp. 135-140, (Mar. 1998)
Arndts, Oral Presentation Abstract, Second International Cell Analysis Products Users Meeting in Hilton Head Island, South Carolina, USA (Jun. 3-5, 1998)
Barr and Plonsey, Biophys. J. 61, 1164-1175 (1992)
Barr and Plonsey, IEEE 42, 1185-11911 (1995)
Cartee and Plonsey, Med & Biol. Eng. & Comp. 30 389-398 (1992)
Cartee and Plonsey, IEEE 39, 76-85 (1992)
Eppich et al., Nature Biotech. 18, 882-887 (2000)
Greenberg, et al. IEEE 46, 505-514 (1999)
Gross, et al. Biophys. J. 50, 339-348 (1986)
Jentsch and Gunther, BioEssays 19, 117-126 (1997)
Klee and Plonsey, Biophys. J. 12 1661-1675 (1972)
Klee and Plonsey, IEEE 23, 347-354 (1976)
Mitchell et al., J. Biomed. Eng. 14, 52-56 (1992)
Plonsey, Med & Biol. Eng. & Comput. 19 311-315 (1981)
March 12, 2021. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
