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Nucleic acid-based detection

Patent 6680377 Issued on January 20, 2004. Estimated Expiration Date: Icon_subject May 12, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Fiber optic apparatus for detecting molecular species by surface enhanced Raman spectroscopy
Patent #: 4781458
Issued on: 11/01/1988
Inventor: Angel ,   et al.

Nucleic acid process containing improved molecular switch
Patent #: 5118801
Issued on: 06/02/1992
Inventor: Lizardi, et al.

Methods for identifying nucleic acid ligands
Patent #: 5270163
Issued on: 12/14/1993
Inventor: Gold, et al.

Surface plasmon resonance sensor and methods for the utilization thereof
Patent #: 5485277
Issued on: 01/16/1996
Inventor: Foster

Systematic evolution of ligands by exponential enrichment: Solution SELEX
Patent #: 5567588
Issued on: 10/22/1996
Inventor: Gold, et al.

Methods and kits for RNA binding compounds
Patent #: 5593835
Issued on: 01/14/1997
Inventor: Rando, et al.

Detection of nucleic acids using G-quartets
Patent #: 5691145
Issued on: 11/25/1997
Inventor: Pitner, et al.

Mach-zehnder type interferometer
Patent #: 5771097
Issued on: 06/23/1998
Inventor: Kusunose, et al.

Devices and methods for detecting nucleic acid analytes in samples
Patent #: 5837466
Issued on: 11/17/1998
Inventor: Lane, et al.

Method for detecting a target molecule in a sample using a nucleic acid ligand
Patent #: 5843653
Issued on: 12/01/1998
Inventor: Gold, et al.

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Inventors

Assignee

Application

No. 09/569960 filed on 05/12/2000

US Classes:

536/22.1, N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.)435/18, Involving hydrolase435/183, ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES435/19, Involving esterase435/6, Involving nucleic acid436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/23.1DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)

Examiners

Primary: Zitomer, Stephanie W.

Attorney, Agent or Firm

International Classes

C12Q 1/68 (20060101)
G01N 21/55 (20060101)
G01N 21/64 (20060101)
G06F 19/00 (20060101)

Abstract

The invention relates to compositions, systems, and methods for simultaneously detecting the presence and quantity of one or more different compounds in a sample using aptamer beacons. Aptamer beacons are oligonucleotides that have a binding region that can bind to a non-nucleotide target molecule, such as a protein, a steroid, or an inorganic molecule. New aptamer beacons having binding regions configured to bind to different target molecules can be used in solution-based and solid, array-based systems. The aptamer beacons can be attached to solid supports, e.g., at different predetermined points in two-dimensional arrays. The invention includes devices, methods, and computer software for carrying out the methods.

Other References

  • Bacher et al., "Nucleic Acid Selection as a Tool for Drug Discovery," Drug Discovery Today, 3(6):265-273 (1998)
  • Cardullo et al., "Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer," Proc. Natl. Acad. Sci. USA, 8:8790-8794 (Dec. 1988)
  • Ciesiolka et al., "Affinity Selection-Amplification from Randomized Ribooligonucleotide Pools," Methods in Enzymology, 267:315-35 (1996)
  • Conrad et al., "Detecting Immobilized Protein Kinase C Isozymes with RNA Aptamers," Analytical Biochemistry, Article No. 0462, 242:261-265 (1996)
  • Conrad et al., "In Vitro Selection of Nucleic Acid Aptamers That Bind Proteins," Methods in Enzymology, 267:336-383 (1996)
  • Davis et al., "Staining of cell surface human CD4 with 2'-F-pyrimidine-containing RNA aptamers for flow cytometry," Nucleic Acids Research, 26(17):3915-3924 (1998)
  • Ellington et al., "In vitro selection of RNA molecules that bind specific ligands," Nature, 346:818-822 (1990)
  • Fitzwater et al., "A SELEX Primer," Methods in Enzymology, 267:275-301 (1996)
  • Gold et al., "Diversity of Oligonucleotide Functions," Annu. Rev. Biochem., 64:763-97 (1995)
  • Gold, "Oligonucleotides as Research, Diagnostic, and Therapeutic Agents," The American Society for Biochemistry and Molecular Biology, Inc., 270(23):13581-13584 (1995)
  • Griffiths et al., The EMBO Journal, 13:3245-3260 (1994)
  • Jayasena, "Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics." Clin. Chem., 45(9):1628-50 (1999)
  • Kramer, "Molecular Beacons: Hybridization Probes for Detection of Nucleic Acids in Homogenous Solutions," http://www.molecular-beacons.com (Dec. 7, 1998)
  • Lashkari et al., "Yeast Microarrays for Genome Wide Parallel Genetic and Gene Expression Analysis," Proc. Nat'l Acad. Sci. USA, 94:13057-62 (1997)
  • Lin et al., "Modified RNA sequence pools for in vitro selection," Nucleic Acids Research, 22(24):5229-5234 (1994)
  • Morgan et al., "A surface plasmon resonance immunosensor based on the streptavidin-biotin complex," Biosensors & Bioelectronics 7:405-410 (1992)
  • Morris et al., "High affinity ligands from in vitro selection: complex targets." Proc. Natl. Acad. Sci., USA, 95(6):2902-7 (1998)
  • Osborne et al., "Aptamers as therapeutic and diagnostic reagents: problems and prospects," Chemical Biology, 1:5-9 (1997)
  • Pagratis et al., "Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor," Nature Biotechnology 15:68-73 (1997)
  • Pease et al., "Light Generated Oligonucleotide Arrays for Rapid DNA Sequence Analysis," Proc. Nat'l Acad. Sci. USA, 91:5022-26 (1994)
  • Potyrailo et al., "Adapting Selected Nucleic Acid Ligands (Aptamers) to Biosensors," Anal. Chem., 70:3419-3425 (1998)
  • Shalon et al., "A DNA Microarray System for Analyzing Complex DNA Samples Using Two-Color Fluorescent Probe Hybridization," Genome Res., 6:639-45 (1996)
  • Schena et al., "Parallel Human Genome Analysis: Microarray-Based Expression Monitoring of 1000 Genes," Proc. Nat'l Acad. Sci. USA, 93: 10514-19 (1996)
  • Sixou et al., "Intracellular Oligonucleotide Hybridization Detected by Fluorescence Resonance Energy Transfer (FRET)," Nucleic Acids Res., 22:662-68 (1994)
  • Tyagi et al., "Molecular Beacons: Probes That Fluoresce Upon Hybridization," Nature Biotech., 14:303-08 (1996)
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