Recombinant DNA method and host cells
Patent 6653101 Issued on November 25, 2003. Estimated Expiration Date: 
November 3, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 3, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesVectors and methods for transformation of eucaryotic cells Transient expression system for producing recombinant protein Transactivation method of regulating foreign gene expression Patent #: 5866359 InventorsAssigneeApplicationNo. 09/704800 filed on 11/03/2000US Classes:435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/358, Chinese hamster ovary (i.e., CHO)435/369, Renal origin or derivative435/372.3, T-cell or derivative435/377, Method of altering the differentiation state of the cell435/455Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cellExaminersPrimary: Ketter, JamesAssistant: Marvich, Maria Attorney, Agent or FirmInternational ClassC12N 15/85 (20060101)Foreign Application Priority Data1989-10-01 GBAbstractThe present invention provides a process and host cell for use in increasing the amount of a desired protein by a cell line. The process and the cell line use a first transcription unit containing a gene for a transactivator protein to control the transactivation of a second transcription unit such that the amount of the desired protein expression can be increased without adversely affecting the cell growth. Preferred transactivator proteins are derived from EIA.Other References
Field of SearchVECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE Recombinant DNA technique included in method of making a protein or polypeptide Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cell Renal origin or derivative Chinese hamster ovary (i.e., CHO) Blood, lymphatic, or bone marrow origin or derivative Method of altering the differentiation state of the cell |
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