Preparation of deallergenized proteins and permuteins
Patent 6639054 Issued on October 28, 2003. Estimated Expiration Date: January 5, 2021. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
530/350, PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES424/171.1, Binds allergen or component thereof435/417, Potato cell or cell line, per se435/418, Plant cell or cell line, per se, is pest or herbicide resistant or pest lethal436/513, INVOLVING IGA, IGD, IGE, OR IGM504/116.1PLANT GROWTH REGULATING COMPOSITIONS (E.G., HERBICIDES, ETC.)
Modified proteins are disclosed that maintain enzymatic and insecticidal activity while displaying reduced or eliminated allergenicity. Epitopes which bind to anti-patatin antibodies were identified, and removed via site directed mutagenesis. Tyrosines were observed to generally contribute to the allergenic properties of patatin proteins. Removal of glycosylation sites was observed to reduce or eliminate antibody binding.Permuteins are also disclosed which have a rearranged amino acid sequence while retaining enzymatic activity.Deallergenized proteins and permuteins can be used as insecticidal materials, as nutritional supplements, and as immunotherapeutic agents.
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January 5, 2021. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
