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Plastid transformation of solanaceous plants

Patent 6541682 Issued on April 1, 2003. Estimated Expiration Date: Icon_subject November 12, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

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Inventors

Assignee

Application

No. 191303 filed on 11/12/1998

US Classes:

800/278, METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART435/417, Potato cell or cell line, per se435/419, Plant cell or cell line, per se, contains exogenous or foreign nucleic acid435/429, Involving potato cell or tissue435/430, Involving regeneration or propagation into a plant or plant part435/468, Introduction of a polynucleotide molecule into or rearrangement of a nucleic acid within a plant cell800/287, The polynucleotide contains a tissue, organ, or cell specific promoter800/288, Nonplant protein is expressed from the polynucleotide800/317, Solanaceae (e.g., eggplant, etc.)800/317.2, Potato800/323.1Petunia

Examiners

Primary: Fox, David T.

Foreign Patent References

  • 2183660 GB. 06/13/1987
  • WO 92/15675 WO. 09/13/1992
  • WO 99/10513 WO. 03/13/1999

International Classes

C12N 015/82
C12N 015/29
C12N 005/10
A01H 005/00
A01H 005/10

Abstract

A method is provided for transforming solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5' to 3' direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilized in the transformation of solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid.

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