Process for the biological production of 1,3-propanediol with high titer

Patent 6514733 Issued on February 4, 2003. Estimated Expiration Date:

August 18, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
Patent #: 5686276
Issued on: 11/11/1997
Inventor: Laffend, et al.

Method for the production of 1,3-propanediol by recombinant microorganisms
Patent #: 6013494
Issued on: 01/11/2000
Inventor: Nakamura, et al.

Method for the recombinant production of 1,3-propanediol Patent #: 6136576
Issued on: 10/24/2000
Inventor: Diaz-Torres, et al.

Inventors

Assignee

E. I. du Pont de Nemours and Company

Application

No. 641652 filed on 08/18/2000

US Classes:

435/158, Polyhydric435/155, Containing hydroxy group435/252.33Escherichia (e.g., E. coli, etc.)

Examiners

Primary: Prouty, Rebecca E.
Assistant: Walicka, Malgorzata A.

Foreign Patent References

3734 764 DE. 10/12/1987
373 230 EP. 02/12/1993
WO 9821339 WO. 05/12/1998
WO9821341 WO. 05/12/1998
WO9928480 WO. 06/12/1999

International Classes

C12P 007/02
C12P 007/18
C12N 001/20

Abstract

The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

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