U.S. patents available from 1976 to present.
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Method for producing recombinant adenovirus

Patent 6485958 Issued on November 26, 2002. Estimated Expiration Date: Icon_subject December 16, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Method of purification of viral vectors
Patent #: 5837520
Issued on: 11/17/1998
Inventor: Shabram, et al.

Defective adenoviruses and corresponding complementation lines
Patent #: 6040174
Issued on: 03/21/2000
Inventor: Imler, et al.

Method for the production and purification of adenoviral vectors Patent #: 6194191
Issued on: 02/27/2001
Inventor: Zhang, et al.

Inventors

Application

No. 202545 filed on 12/16/1998

US Classes:

435/239, Recovery or purification435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)

Examiners

Primary: Mosher, Mary E.

Attorney, Agent or Firm

Foreign Patent References

  • WO 94/28152 WO. 12/20/1994
  • WO 95/19427 WO. 07/20/1995
  • WO 95/23867 WO. 09/20/1995
  • WO 95/25789 WO. 09/20/1995

International Classes

C12N 007/02
C12N 007/01

Foreign Application Priority Data

1996-07-01 FR

Abstract

The invention concerns a method for producing recombinant adenovirus by which viral DNA is introduced in a packaging cell culture and the viruses produced are harvested after liberation in the supernatant. The invention also concerns the viruses produced and their use.

Other References

  • Van Der Vliet, P.C., et al., "Thermolabile DNA Binding Proteins from Cells Infected with a Temperature-Sensitive Mutant of Adenovirus Defective in Viral DNA Synthesis," Journal of Virology, 15:2 pp. 348-354 (1975)
  • Huyghe et al., Purification of a Type 5 Recombinant Adenovirus Encoding Human p53 by Column Chromatography, Human Gene Therapy 6:1403-1416 (1995)
  • O'Neil et al., Virus Harvesting and Affinity-Based Liquid Chromatography, Bio/Technology 11, 173-178 (1993
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