Reagants and methods for immobilized polymer synthesis and display
Nucleic acid probes chemically modified at 5'(OH) and/or at 3'(OH) for the purpose of introducing one or more non-radioactive marking elements at these sites, and method for preparing the same
Gene synthesis method
Oligonucleotides having chiral phosphorus linkages Patent #: 6239265
ApplicationNo. 486241 filed on 05/12/2000
US Classes:435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)530/334, Polymer supported synthesis, e.g., solid phase synthesis, Merrifield synthesis, etc.536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.33, Primers536/25.3Synthesis of polynucleotides or oligonucleotides
ExaminersPrimary: Fredman, Jeffrey
Assistant: Chakrabarti, Arun Kr.
Attorney, Agent or Firm
Foreign Patent References
International ClassesC12P 019/34
Foreign Application Priority Data1997-08-22 DE
AbstractThe conventional synthesis of nucleic acid polymers from several oligonucleotides comprises several cycles of intermediate product synthesis, purification of the intermediate products and synthesis of a full length product (up to a maximum length of approximately 1000 nucleotides). The novel method shall be carried out in one reaction step and result in nucleic acid polymers of more than 1000 nucleotides.According to the invention, two or more linkable oligonucleotides are provided that in a continuous arrangement and after linkage can form a primary strand, and one or more non-linkable oligonucleotides are provided, each of the non-linkable oligonucleotides comprising two adjoining regions, the first of which is complementary to the 3' end of a linkable oligonucleotide and the second of which is complementary to the 5' end of a further linkable oligonucleotide. The linkable oligonucleotides are hybridized with the complementary regions of the non-linkable oligonucleotides and then linked enzymatically, chemically or photochemically.The method is suited for the de novo synthesis of long nucleic acid chains.
Field of SearchInvolving nucleic acid
Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)
Acellular exponential or geometric amplification (e.g., PCR, etc.)
Synthesis of polynucleotides or oligonucleotides
DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)
Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA
Polymer supported synthesis, e.g., solid phase synthesis, Merrifield synthesis, etc.