Method for the preparation of a probe for nucleic acid hybridization
Patent 6461871 Issued on October 8, 2002. Estimated Expiration Date: 
April 5, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
April 5, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod of making oligonucleotides and oligonucleotide analogs using phospholanes and enantiomerically resolved phospholane analogues Patent #: 5646267 InventorsAssigneeApplicationNo. 486853 filed on 04/05/2000US Classes:436/94, Saccharide (e.g., DNA, etc.)435/6, Involving nucleic acid435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.33PrimersExaminersPrimary: Whisenant, EthanAssistant: Lu, Frank Attorney, Agent or FirmForeign Patent References
International ClassesG01N 033/00C12Q 001/68 C12P 019/34 C07H 021/02 C07H 021/04 Foreign Application Priority Data1997-05-09 SEAbstractThe invention relates to a method for preparing a probe, thus prepared probe, and the use of such a probe for selectively choosing sequence for nucleic acid diagnostic purposes, using preferably homogenous solutions. The invention is based upon a great number of probes having different sequences and lengths which all are complementary to different parts of the nucleic acid to be detected, which probes are synthetised on a solid matrix. The signal which they provide in non-hybridized condition is monitored, whereupon the nucleic acid to be detected is added, and the signal is monitored again. Those probes that show the most significant difference in signal are those, from a sensitivity point of view, that are the most suitable one.Other References
Field of SearchInvolving nucleic acidPolynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Acellular exponential or geometric amplification (e.g., PCR, etc.) ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES APPARATUS Including measuring or testing Measuring or testing for antibody or nucleic acid, or measuring or testing using antibody or nucleic acid Including optical measuring or testing means BIOSPECIFIC LIGAND BINDING ASSAY Saccharide (e.g., DNA, etc.) DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Primers Synthesis of polynucleotides or oligonucleotides Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.) |
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