Organic semiconductor recognition complex and system
Patent 6303316 Issued on October 16, 2001. Estimated Expiration Date: June 30, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/6, Involving nucleic acid435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)436/94, Saccharide (e.g., DNA, etc.)536/23.1DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)
In a recognition complex system, nucleic acid ligands comprising random DNA sequences are operatively coupled to an organic semiconductor and distributed so as to form an array of recognition complexes. When an unknown chemical or biological analyte is applied to the array, the electrical and/or photochemical properties of one or more of the recognition complexes are altered upon binding of the nucleic acid ligand to the analyte. The degree to which the electrical and/or photochemical properties change is a function of the affinity of the nucleic acid ligand sequence for the analyte. The electrical and photochemical changes associated with the array, as a whole, can be used as a unique signature to identify the analyte. In certain embodiments, an iterative process of selection and amplification of nucleic acid ligands that bind to the analyte can be used to generate a new array with greater affinity and specificity for a target analyte, or to produce one or more nucleic acid ligands with high binding affinity for an analyte. The present invention also provides methods for preparing nucleic acid ligands that bind with high affinity to an analyte and using such nucleic acid ligands to neutralize the analyte.
Other References
Bruno & Yu, "Immunomagnetic-electrochemiluminescent detection of Bacillus anthracis spores in soil matrices", Appl. Environ. Microbio., 62:3474-76, 1996
Bruno et al., "Preliminary electrochemiluminescence studies of metal ion-bacterial diazoluminomelanin (DALM) interactions", J. Biolumin, Chemilum., 13:117-123, 1998
Bruno, John G., "In vitro selection of DNA to chloroaromatics using magnetic microbead-based affinity separation and fluorescence detection", Biochim. Biophys. Res. Comm., 234:117-120, 1997
Bruno, John G., "A colorimetric inhibition study of single-stranded DNA decamer sequence interactions with dinitrotoluene", Biochem. and Biophy Res. Comm., 236:344-346, 1997
Bruno, John G., "Broad applications of electrochemiluminescence technology to the detection and quantitation of microbiological, biochemical and chemical analytes", Recent Res. Devel. in Microbiology, 1:25-46, 1997
Famulok and Mayer, "Aptamers as Tools in Molecular Biology and Immunology", Curr. Topics in Microb. and Immun., 243:123-136, 1999
Jayasena, S.D., "Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics", Clin. Chem., 34:1628-1650, 1999
Kiel, Johnathan et al., "Diazoluminomelanin: A Synthetic Electron and Nonradiative Transfer Biopolymer", Proceedings of the 1989 International Symposium on Charge and Field Effects in Biosystems-2. Held Jun 4-9, 1989 in Richmond, Virginia, pp. 293-300, M.J. Allen et al., Editors
Yu and Bruno, "Immunomagnetic-electrochemi-luminescent detection of Escherichia coli 0157 and Salmonella typhimurium in foods and environmental water samples", Appl. Environ. Microbiol., 62:587-92, 1996
Bruno & Yu, "Immunomagnetic-electrochemiluminescent detection of Bacillus anthracis Spores in Soil Matrices", Appl. and Environ. Microb., 62(9):3474-3476, 1996
Drolet, D.W. et al., A high throughput platform for systematic evolution of ligands exponential enrichment (SELEX), Comb. Chem. High Throughput Screen, Oct. 2(5):271-278, 1999
Ellington & Szostak, "In vitro selection of RNA molecules that bind specific ligands", Nature, 346:818-822, 1990
Ellington & Szostak, "Selection in vitro of single straded DNA molecules that fold into specific ligand-binding structures", Nature, 355:850-52, 1992
Kiel, J.L. et al., "Diazoluminomelanin: a synthetic electron and nonradiative transfer biopolymer", In Charge and Field Effects in Biosystems-2, J.J. Allen, S.F. Cleary, F.M. Hawkridge, editors; Plenum Press, New York, 1989
Kiel, J.L. et al., "Diazoluminomelanin: a synthetic luminescent biopolymer", Free Radic. Res. Commun., 8(2):115-21, 1990
Klug and Famulok, "All you wanted to know about SELEX", Mol. Biol. Reports, 20:97-107, 1994
Kugler et al., "Photoelectron spectroscopy and quantum chemical modeling applied to polymer surfaces and interfaces in light-emitting devices", Accounts of Chemical Research, 32:225-234, 1999
Tuerk, "In vitro evolution of functional nucleic acids: high-affinity RNA ligands of HIV-1 proteins", Gene 137:33-39, 1993
Tuerk, "Using the SELEX combinatorial chemistry process to find high affinity nucleic acid ligands to target molecules", Meth. Mol. Biol. 67:219-30, 1997
Gatto-Menking et al., "Sensitive detection of biotoxoids and bacterial spores using an immunomagnetic electrochemiluminescence sensor", Biosensors & Bioelectronics, 10:501-507, 1995
Hacia et al., "Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two-colour fluorescence analysis", Nature Genetics, 14:441-447, 1996
Brody, E.N. et al., "The use of Aptamers in large arrays for molecular diagnostics", Mol. Diagn., Dec., 4(4):381-388, 1999
Lorsch and Szostak, "In vitro selection of nucleic acid sequences that bind small molecules." In: Combinatorial Libraries: Synthesis Screening and Application Potential (R. Cortese, ed.), Walter de Gruyter Publishing Co., New York, pp. 69-86, 1996
Shoemaker et al., "Quantitative phenotypic analysis of yeast deletion mutants using ahighly parallel molecular bar-coding strategy", Nature Genetics, 14:450-456, 1996
Tuerk, C. and Gold, L., "Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA Polymerase", Science, 249:505-510, 1990
Yu and Bruno, "Immunomagnetic-electrochemiluminescent detection of Escheria coli O157 and Salmonella typhimurium in Foods and Environmental Water Samples", App. and Environ. Microbiology, 62(2):587-592, 199
June 30, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
