Transgenic seedless fruit comprising AGL or GH3 promoter operably linked to isopentenyl transferase or tryptophan monooxygenase coding DNA
Patent 6268552 Issued on July 31, 2001. Estimated Expiration Date: 
May 6, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
May 6, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesOvary-tissue transcriptional factors Transgenic plants belonging to the species Cucumis melo Enhanced insect resistance in plants genetically engineered with a plant hormone gene involved in cytokinin biosynthesis Patent #: 5496732 InventorAssigneeApplicationNo. 073587 filed on 05/06/1998US Classes:800/317.4, Tomato435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)800/278, METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART800/284, The polynucleotide alters carbohydrate production in the plant800/298, Higher plant, seedling, plant seed, or plant part (i.e., angiosperms or gymnosperms)800/307, Cucumber800/308WatermelonExaminersPrimary: Nelson, Amy J.Attorney, Agent or FirmForeign Patent References
International ClassesA01H 005/00A01H 005/10 C12N 015/82 AbstractThe present invention provides methods and DNA constructs for the genetic engineering of plant cells to produce plants which produce substantially seedless fruit in the absence of exogenous growth factors (auxins or cytokinins) and in the absence of pollination. The substantially seedless fruits produced by the methods described herein are about the size of wildtype seeded fruit (or somewhat larger) and these fruits are equal to or superior to the wildtype seeded fruit with respect to solid content and flavor. The seedless fruits of the present invention are produced in transgenic plants which contain and express auxin or cytokinin biosynthetic genes, e.g., tryptophan oxygenase or isopentenyl transferase coding sequences expressed under the regulatory control of GH3 or AGL promoter sequences directing preferential or tissue specific expression of a downstream gene in the ovaries or developing fruit.Other References
Field of SearchRecombinant DNA technique included in method of making a protein or polypeptideVECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) Plant cell or cell line, per se, contains exogenous or foreign nucleic acid Introduction of a polynucleotide molecule into or rearrangement of a nucleic acid within a plant cell Encodes an enzyme Encodes a plant polypeptide METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART The polynucleotide contains a tissue, organ, or cell specific promoter Higher plant, seedling, plant seed, or plant part (i.e., angiosperms or gymnosperms) Cucumber Watermelon Tomato Tobacco Apple Citrus (e.g., orange, lemon, lime, etc.) Squash (e.g., pumpkin, zucchini, etc.) Potato Chrysanthemum Solanaceae (e.g., eggplant, etc.) Pepper The polynucleotide alters carbohydrate production in the plant |
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