Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
August 11, 2020. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesNucleotide hybridization assay for protozoan parasites Hybridization method for the detection of genetic material Nucleic acid process containing improved molecular switch Methods for identifying nucleic acid ligands Capture sandwich hybridization method and composition Assays and kits incorporating nucleic acid probes containing improved molecular switch Methods for detecting and assaying nucleic acid sequences Detection of nucleic acids using G-quartets Method of identifying a stochastically-generated peptide, polypeptide, or protein having ligand binding property and compositions thereof Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system InventorsAssigneeApplicationNo. 581326 filed on 08/11/2000US Classes:435/6, Involving nucleic acid436/94Saccharide (e.g., DNA, etc.)ExaminersPrimary: Horlick, Kenneth R.Attorney, Agent or FirmForeign Patent References
International ClassC12Q 001/68AbstractA method for detecting a target molecule in a test mixture suspected of containing said target molecule is described. The nucleic acid ligand capable of binding to the target molecule has a first sequence A and a second sequence B, which are partially complementary sequences that form an imperfect intramolecular duplex, which unwinds upon the binding of the target to the nucleic acid ligand. Sequences A and B are able to participate in extramolecular hybridization reactions only when the duplex is unwound. Three different cascade nucleic acids contain a first sequence and a second sequence, which are partially complementary sequences. At least one sequence is exactly complementary to A or B. The second sequence may be complementary to A or B, or may be a third sequence C, or its complement. The test mixture suspected of containing the target molecule is contacted with the nucleic acid ligand, causing the duplex of the nucleic acid ligand to unwind such that sequences A and B become available for extramolecular hybridization. This mixture is contacted with the first, second, and third cascade nucleic acids so that the unpaired A and B sequences triggers a cascade of intermolecular hybridization involving the cascade nucleic acids in which intermolecular hybridization takes place between A and its complement, B and its complement, and between C its complement, leading to the formation of a multimolecular hybridization complex. The presence of the multimolecular hybridization complex is then detected.Other References
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