Universal primer sequence for multiplex DNA amplification
Patent 6207372 Issued on March 27, 2001. Estimated Expiration Date: 
February 10, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
February 10, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod for amplifying unknown nucleic acid sequences Nucleotide sequence encoding the enzyme I-SceI and the uses thereof Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease Universal primer sequence for multiplex DNA amplification Patent #: 5882856 InventorAssigneeApplicationNo. 983466 filed on 02/10/1998US Classes:435/6, Involving nucleic acid435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Jones, W. GaryAssistant: Tung, Joyce Foreign Patent References
International ClassesC12Q 1/6/87/ C12P 19//34 7/ C07H 21//04 AbstractThe present invention provides primer that allow simultaneous amplification of multiple DNA target sequences present in a DNA sample. Further provided are methods for detecting multiple defined target DNA sequences in a DNA sample. Methods for high-throughput genetic screening are also provided. In yet another aspect, the present invention provides single-stranded oligonucleotide DNA primers for amplification of a target DNA sequence in a multiplex polymerase chain reaction.Other References
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