Patent 6200806 Issued on March 13, 2001. Estimated Expiration Date: June 26, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/366, Human435/325ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE
A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (-); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
Other References
Andrews, P., et al., "Cell lines from human germ cell tumours," Teratocarcinoms and Embryonic Stem Cells; A Practical Approach, Oxford: 1RL Press, Ch. 8:207-248 (1987)
Andrews, P., et al., "Pluripotent Embryonal Carcinoma Clones Derived from the Human Teratocarcinoma Cell Line Tera-2," Lab. Invest., 50(2):147-162 (1984)
Bongso, A., et al., "Isolation and culture of inner cell mass cells from human blastocysts," Human Reprod., 9(1):2110-2117 (1994)
Bongso, A., et al., "The Growth of Inner Cell Mass Cells from Human Blastocysts," Theriogenology, 41:167 (1994)
Brown, D.G., et al., "Criteria that Optimize the Potential of Murine Embryonic Stem Cells for In Vitro and In Vivo Developmental Studies," In Vitro Cell. Dev. Bio., 28(A)773-778 (Nov., Dec. 1992)
Damjanov, Ivan., et al., Retinoic Acid-Induced Differentiation of the Developmentally Pluripotent Human Germ Cell Tumor-Derived Cell Line, NCCIT, Laboratory Investigation, 68(2):220-232 (1993)
Doetschman, T., et al., Establishment Of Hamster Blastocyst-Derived Embryonic Stem (ES) Cells, Developmental Biology, 127:224-227 (1988)
Doetschman, T., et al., "The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium," J. Embryol. exp. Morph., 87:27-45 (1985)
Evans, M., et al., "Establishment in culture of pluripotential cells from mouse embryos," Nature, 292:154-156 (1981)
Evans, M., et al., "Derivation and Preliminary Characterization of Pluripotent Cell Lines from Porcine and Bovine Blastocysts," Theriogenology, 33(1):125-128 (1990)
Giles, J., et al., "Pluripotency of Cultured Rabbit Inner Cell Mass Cells Detected by Isozyme Analysis and Eye Pigmentation of Fetuses Following Injection into Blastocysts or Morulae," Mol. Reprod. Dev., 36:130-138 (1993)
Golos, T., et al., "Cloning of Four Growth Hormone/Chorionic Somatomammotropin-related Complementary Deoxyribonucleic Acids Differentially Expressed during Pregnancy in the Rhesus Monkey Placenta," Endocrinology, 133(4):1744-1752 (1993)
Graves, K., et al., "Derivation and Characterization of Putative Pluripotential Embryonic Stem Cells from Preimplantation Rabbit Embryos," Mol. Reprod. Dev., 36:424-433 (1993)
Lapidot, T., et al., "Modeling Human Hematopoiesis in Immunodeficient Mice," Lab. Animal Sci., 43(2):147-149 (1993)
Marshall, E., "Rules on Embryo Research Due Out," Science, 265:1024-1026 (1994)
Notarianni, E., et al., "Maintenance and differentiation in culture of pluripotential embryonic cell lines from pig blastocysts," J. Reprod. Fert. Suppl., 41:51-56 (1990)
Notarianni, E., et al., "Derivation Of Pluripotent, Embryonic Cell Lines From The Pig And Sheep," J. Rep. & Fert. 43 255-260 (1991)
Piedrahita, et al., "On The Isolation Of Embryonic Stem Cells: Comparative Behavior Of Murine, Porcine And Ovine Embryos," Theriogenology, 34(5):879-901 (1990)
Piedrahita, J. Dissertation On "Studies On The Isolation Of Embryonic Stem (ES) Cells: Comparative Behavior Of Murine, Porcine, And Ovine Species," University of California Davis, 1989
Rossant, J., et al., "The relationship between embryonic, embryonal carcinoma and embryo-derived stem cells," Cell Diff., 15:155-161 (1984)
Seshagiri, P., et al., "Non-Surgical Uterine Flushing for the Recovery of Preimplantation Embryos in Rhesus Monkeys: Lack of Seasonal Infertility," Am. J. Primatol., 29:81-91 (1993)
Strojek, R. et al., "A Method For Cultivating Morphologically Undifferentiated Embryonic Stem Cells From Porcine Blastocysts," Theriogenology 33 901-913 (1990
Sukoyan, M., et al., "Isolation and Cultivation of Blastocyst-derived Stem Cell Lines from American Mink (Mustela vision)," Mol. Reprod. Dev., 33:418-431 (1992)
Sukoyan, M., et al., "Embryonic Stem Cells Derived from Morulae, Inner Cell Mass, and Blastocysts of Mink: Comparisons of Their Pluripotencies," Mol. Reprod. Deve., 36:148-158 (1993)
Talbot, et al. "Culturing The EpiBlast Cells Of The Pig Blastocyst", , In Vitro Cell. Dev. Bio., 29(A):543-554 (1993)
Thomson, J., et al., "Nonsurgical uterine stage preimplantation embryo collection from the common marmoset," J. Med. Primatol., 23:333-336 (1994)
Thomson, James A., et al., "Pluripotent Cell Lines Derived from Common Marmoset (Callithrix jacchus) Blastocysts," Biology of Reproduction, 55:254-259 (1996)
Ware, et al., "Development Of Embryonic Stem Cell Lines From Farm Animals", Biol. Reprod., 38(Suppl. 1):129 (1988)
Wenk, J., et al., "Glycolipods of Germ Cell Tumors: Extended Globo-series Glycolipods are a Hallmark of Human Embryonal Carcinoma Cells," Int. J. Can, 58:108-115 (1994)
Williams, R., et al., "Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells," Nature, 336:684-692 (1988)
Thomson et al. Embryonic Stem Cell Lines Derived from Human Blastocysts. Science, vol. 282, pp. 1145-1147, Nov. 6, 1998
Cruz et al. Origin of Embryonic and Extraembryonic Cell Lineages in Mammalian Embryos. Current Communications, vol. 4, pp. 147-204, 1991
Nichols et al. Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. Development, vol. 110, pp. 1341-1348, 1990
Clark et al. Germ line manipulation: applications in agriculture and biotechnology. Transgenic Animals. Grosveld et al. eds. p. 250, 199
June 26, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
