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US Patent 6172040 - Immobilized lactoferrin antimicrobial agents and the use thereof

US Patent Issued on January 9, 2001
Estimated Patent Expiration Date: Icon_subject May 28, 2019Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
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Abstract

Disclosed is a method for treating products, such as meat products, with immobilized lactoferrin to reduce microbial contamination. The lactoferrin is immobilized on a naturally occurring substrate, preferably a galactose-rich polysaccharide. In some embodiments, the lactoferrin is applied as an aqueous solution containing a mixture of the immobilized lactoferrin and native lactoferrin, and a buffer system that includes a physiologically acceptable acid, such as citric acid, a physiologically acceptable base, such as sodium bicarbonate, and a physiologically acceptable salt, such as sodium chloride.

Other References

  • Harper et al., Dairy Technology and Engineering. Westpont: The Avi Publ. Co., Inc. pp. 20-23, 28-37 1976.
  • Naidu, A.S. et al. "Milk lactoferrin--natural microbial blocking agent (MBA) for food safety",Environ, & Nutri. Interac., 2: 35-50, 1998
  • Naidu, A.S. et al., "Influence of Lactoferrin on Host--Microbe Interactions", Lactoferrin: Interactions and Biological Functions, Chapter 17, edited by T.W. Hutchins and B. Lonnerdal, Humana Press Inc. Totowa, NJ, pp. 259-275, 1997
  • Alugupalli, K.R. et al., "Lactoferrin interaction with Actinobacillus actinomycetemcomitans", Oral Microbiol. Immunol., 10: 35-41, 1995
  • Naidu, A.S. et al., "Lactoferrin Interaction with Salmonellae Potentiates Antibiotic Susceptibility in vitro", Diagn. Microbiol. Infect. Dis., 20: 69-75, 1994
  • Alugupalli, K.R. et al., "Effect of lactoferrin on interaction of Prevotella intermedia with plasma and subepithelial matrix proteins", Oral Microbiol. Immunol., 9: 174-179, 1994
  • Gerlach, D. et al., "Identification of a Novel Lectin in Streptococcus pyrogenes and Its Possible Role in Baterial Adherence to Pharyngea Cells", Current Microbiology, 28: 331-338, 1994
  • Erdei, J. et al., "Lactoferrin Binds to Porins OmpF and OmpC in Escherichia coli", Infection and Immunity, p. 1236-1240, Apr. 1994
  • Paulsson, M.A. et al. "Thermal Behavior of Bovine Lactoferrin in Water and Its Relation to Bacterial Interaction and Antibacterial Activity", J. Dairy Sci., 76:3711-3720, 1993
  • Naidu, S.S. et al., "Relationship between Antibacterial Activity and Porin Binding of Lactoferrin in Eschericha coli and Salmonella typhimurium", Antimicrobial Agents and Chemotherapy, 37(2): 240-245, Feb. 1993
  • Tigyi, Z. et al., "Lactoferrin-Binding Proteins in Shigella flexneri", Infection and Immunity, 60(7): 2619-2626, Jul. 1992
  • Naidu, A.S. et al., "Indentification of a human lactoferrin-binding protein in Staphylococcus aureus", J. Med. Microbiol., 36: 177-183, 1992
  • Kishore, A.R. et al., "Detection of bacterial interaction with lactoferrin by an enzyme-linked ligand binding assay (ELBA)", J. Med. Microbiol., 37: 341-345, 1992
  • Kalfas, S. et al., "Laminin binding to Prevotella intermedia", Oral Microbiol. Immunol., 7: 235-239, 1992
  • Gado, I. et al., "Correlation between Human Lactoferrin Binding and Colicin Susceptibility in Escherichia coli", Antimicrobial Agents and Chemotherapy, 35(12): 2538-2543, Dec. 1991
  • Kishore, A.R. et al., "Specific binding of lactoferrin to Aeromonas hydrophila", FEMS Microbiology Letters, 83:115-120, 1991
  • Kalfas, S. et al., "Human lactoferrin binding to Porphyromonas gingivalis, Prevotella intermedia and Prevotella melaninogenica", Oral Microbiol. Immunol., 6: 350-355, 1991
  • Naidu, A.S. et al., "Human lactoferrin binding in clinical isolates of Staphylococcus aureus", J. Med. Microbiol., 34: 323-328, 1991
  • Naidu, S.S. et al., "Specific binding of lactoferrin to Escherichia coli isolated from human intestinal infections", APMIS, 99: 1142-1150, 1991
  • Naidu, A.S. et al., "Bovine Lactoferrin Binding to Six Species of Coagulase-Negative Staphylococci Isolated from Bovine Intramammary Infections", J. Clinical Microbiol., 28(10): 2312-2319, 199

Inventor

Assignee

Application

No. 322700 filed on 05/28/1999

US Classes:

514/6, Heavy metal containing (e.g., hemoglobin, etc.)422/28, Using disinfecting or sterilizing substance422/32, Treating bulk material426/310, Preserving426/322, Treatment with heavy metal or compound thereof426/326, With biocidal or disinfecting chemical agent426/332, Animal flesh426/335, Biocidal or disinfecting chemical agent426/532, With biocide or biostat514/8, Glycoprotein (carbohydrate containing)514/21Produced by or extracted from animal tissue

Field of Search

424/439, Food or edible as carrier for pharmaceutical424/442, Animal food426/302, SURFACE COATING OF A SOLID FOOD WITH A LIQUID426/310, Preserving426/321, INHIBITING CHEMICAL OR PHYSICAL CHANGE OF FOOD BY CONTACT WITH A CHANGE INHIBITING CHEMICAL AGENT OTHER THAN AN ANTIOXYGEN AGENT426/322, Treatment with heavy metal or compound thereof426/326, With biocidal or disinfecting chemical agent426/332, Animal flesh426/335, Biocidal or disinfecting chemical agent426/532, With biocide or biostat426/574, Meat, meat extracts, or meat analog426/652, For use with meat, poultry or seafood422/28, Using disinfecting or sterilizing substance422/32, Treating bulk material514/6, Heavy metal containing (e.g., hemoglobin, etc.)514/8, Glycoprotein (carbohydrate containing)514/21, Produced by or extracted from animal tissue530/395, Glycoprotein, e.g., mucins proteoglycans, etc.530/400, Metal containing, e.g., chromoproteins, ferritin, ferredoxins, etc.530/810, CARRIER - BOUND OR IMMOBILIZED PEPTIDES OR PROTEINS AND THE PREPARATION THEREOF, E.G., BIOLOGICAL CELL OR CELL FRAGMENT AS CARRIER, ETC.530/811, Peptides or proteins is immobilized on, or in, an inorganic carrier530/812, Peptides or proteins is immobilized on, or in, an organic carrier530/813, Carrier is a saccharide530/814, Cellulose or derivatives thereof530/815, Carrier is a synthetic polymer530/816, Attached to the carrier via a bridging agent530/817Entrapped within the carrier, e.g., gel, hollow fibre, etc.

Examiners

Primary: Russel, Jeffrey E.

Attorney, Agent or Firm

US Patent References

4667018, Process for the purification of proteins using acidic polysaccharide gels
Issued on: 05/19/1987
Inventor: Prieels ,   et al.
4791193, Process for producing bovine lactoferrin in high purity
Issued on: 12/13/1988
Inventor: Okonogi ,   et al.
4946944, Process for the selective extraction of metalloproteins from whey by adsorption and elution
Issued on: 08/07/1990
Inventor: Frankinet, et al.
5015487, Use of lanthionines for control of post-processing contamination in processed meat
Issued on: 05/14/1991
Inventor: Collison, et al.
5147853, Infection protectant
Issued on: 09/15/1992
Inventor: Dosako, et al.
5156875, Stabilized antimicrobial food composition
Issued on: 10/20/1992
Inventor: Monte
5179197, Secretory component-containing composition
Issued on: 01/12/1993
Inventor: Uchida, et al.
5214028, Lactoferrin hydrolyzate for use as an antibacterial agent and as a tyrosinase inhibition agent
Issued on: 05/25/1993
Inventor: Tomita, et al.
5296464, Bioactive agents and compositions for materials comprising the bioactive agent and a method for treating materials therewith
Issued on: 03/22/1994
Inventor: Tomita, et al.
5389611, Lactoferrin hydrolyzate for use as an antibacterial agent and as a tyrosinase inhibition agent
Issued on: 02/14/1995
Inventor: Tomita, et al.
5543392, Digestive tract cell activating agent of EGF and lactoferrin
Issued on: 08/06/1996
Inventor: Tomita, et al.
5573801, Surface treatment of foodstuffs with antimicrobial compositions
Issued on: 11/12/1996
Inventor: Wilhoit
5606086, Iron lactoferrin complexes and preparation thereof
Issued on: 02/25/1997
Inventor: Dosako, et al.
5656591, Antimicrobial agents and method for treating products therewith
Issued on: 08/12/1997
Inventor: Tomita, et al.
5834424Use of lactoferrin for therapy of acute of chronic infectious diseases by the intracellular gram-positive pathogens streptococcus pyogenes and staphylococcus aureus
Issued on: 11/10/1998
Inventor: Valenti, et al.

Foreign Patent References

  • 568200 EP. 11/20/1993
  • 2099065 RU. 12/20/1997

International Classes

A23B 004/20
A23L 003/349.9
A23L 003/352.6
A61K 038/40
A61L 002/18

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