Method of enriching for mammalian stem cells

Patent 6146888 Issued on November 14, 2000. Estimated Expiration Date:

December 29, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Inventors

Assignee

The University of Edinburgh

Application

No. 535141 filed on 12/29/1995

US Classes:

435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE435/350, Canine cell, per se435/351, Feline cell, per se435/352, Rodent cell, per se435/353, Rat (i.e., Rattus)435/354, Mouse (i.e., Mus)435/355, Blood or lymphatic origin or derivative435/363, Primate cell, per se435/366, Human435/368, Nervous system origin or derivative435/372Blood, lymphatic, or bone marrow origin or derivative

Examiners

Primary: Crouch, Deborah
Assistant: Wilson, Michael C.

Attorney, Agent or Firm

Finnegan, Henderson, Farabow, Garrett & Dunner

Foreign Patent References

0 235 113 EP. 09/12/1987
WO 90/01541 WO. 02/12/1990
WO 91/01140 WO. 02/12/1991
WO 92/11355 WO. 07/12/1992

International Classes

C12N 015/85
C12N 015/86

Foreign Application Priority Data

1993-04-21 GB

Abstract

Mammalian stem cells are obtained and maintained in vitro whose genome has at least one nucleic acid construct encoding an antibiotic resistance gene operatively linked to a promoter specific for mammalian stem cells. The preferential expression of the antibiotic resistance gene in the stem cells results in the preferential survival of the stem cells in the presence of antibiotic.

Other References

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