High level expression of proteins
Patent 6114148 Issued on September 5, 2000. Estimated Expiration Date: 
September 20, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 20, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesManufacture and expression of structural genes Codon pair utilization Cancer-associated SCM-recognition factor, preparation and method of use Phosphinothricin-resistance gene, and its use Cloned genes encoding reverse transcriptase lacking RNase H activity Bovine basic fibroblast growth factor Overexpression of mammalian and viral proteins Patent #: 5786464 InventorsAssigneeApplicationNo. 717294 filed on 09/20/1996US Classes:435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/69.6, Blood proteins435/91.4, Modification or preparation of a recombinant DNA vector435/91.41, By insertion or addition of one or more nucleotides435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE435/440PROCESS OF MUTATION, CELL FUSION, OR GENETIC MODIFICATIONExaminersPrimary: Guzo, DavidAssistant: Shuman, Jon Attorney, Agent or FirmForeign Patent References
International ClassesC12N 015/11C12P 015/40 C12P 019/34 AbstractThe invention features a synthetic gene encoding a protein normally expressed in a mammalian cell wherein at least one non-preferred or less preferred codon in the natural gene encoding the protein has been replaced by a preferred codon encoding the same amino acid.Other References
Field of SearchEncodes an animal polypeptideViral protein Recombinant DNA technique included in method of making a protein or polypeptide Acellular preparation of polynucleotide Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.) VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) |
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