Screen for ultraspiracle inhibitors
Patent 6110698 Issued on August 29, 2000. Estimated Expiration Date: 
May 30, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
May 30, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesPolynucleotides encoding insect steroid hormone receptor polypeptides and cells transformed with same Isolated nucleic acid encoding a ubiquitous nuclear receptor Insect retinoid-like receptor compositions and methods Nucleic acids encoding peroxisome proliferator-activated receptor Patent #: 5861274 InventorsAssigneeApplicationNo. 865960 filed on 05/30/1997US Classes:435/29, Involving viable micro-organism435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/255.1, Yeast435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE435/375, Method of regulating cell metabolism or physiology530/350, PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES536/23.1DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)ExaminersPrimary: Kemmerer, ElizabethAssistant: Basi, Nirmal S. Attorney, Agent or FirmInternational ClassesC12Q 001/02C12N 015/63 C12N 015/81 C12N 015/00 AbstractThis invention discloses methods for identifying compounds, variant nuclear proteins and other auxiliary proteins that interfere with the Drosophila ultraspiracle protein ("Usp") and homologs thereof. The methods disclosed involve transformed yeast cells which contain a Usp binding partner, Usp or a homolog thereof which can bind with the Usp binding partner, and a reporter gene which requires a functional Usp--Usp binder partner complex for expression. The transformed yeast cells are incubated in the presence of a test compound to form a test culture and in the absence of a test compound to form a control culture. The expression of the reporter gene is monitored in one example by exposing the test and control cultures to canavanine under conditions in which control cultures exhibit reduced growth and detecting test cultures in which growth is increased relative to growth of control cultures.Other References
Field of SearchInvolving viable micro-organismInvolving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP Recombinant DNA technique included in method of making a protein or polypeptide ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE Method of regulating cell metabolism or physiology Yeast VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) |
| ||||||||||||||
