U.S. patents available from 1976 to present.
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Use of selective DNA fragment amplification products for hybridization-based genetic fingerprinting, marker assisted selection, and high-throughput screening

Patent 6100030 Issued on August 8, 2000. Estimated Expiration Date: Icon_subject January 9, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

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Inventors

Assignee

Application

No. 005205 filed on 01/09/1998

US Classes:

435/6, Involving nucleic acid435/91.2Acellular exponential or geometric amplification (e.g., PCR, etc.)

Examiners

Primary: Campbell, Eggerton A.

International Classes

C12Q 001/68
C12P 019/34

Abstract

Methods of genotyping amplified mixtures of DNAs, nucleic acid markers and methods of obtaining markers, kits, recombinant plants, positional cloning and integrated systems for making genotypes and assessing hybridizations are provided. These features are applicable to DNA fingerprinting, marker assisted selection, genotyping, cladistic analysis of variance, and high throughput laboratory screening methods.

Other References

  • Howel et al. Europ. J. Immunogenet. 20:363-371, 1993
  • Schlayer et al. J. Virol.Meth. 38:333-341 1992
  • Villard et al. Am.J.Hum.Genet. 58:1268-1278 Jun. 1996
  • Bej et al., "Amplification of Nucleic Acids by Polymerase Chain Reaction (PCR) and other Methods and their Applications", Critical Reviews, in Biochemistry and Molecular Biology, 26:3-4 301-334 (1991)
  • Penner et al., "Rapid RAPD screening of plant DNA using dot blot hybridization", Molecular Breeding, 2:1 7-10, see entire article (1996)
  • Austin et al., "Genetic resolution and verification of quantitative trait loci for flowering and plant height with recombinant inbred lines of maize1 ", Genome 39:5 957-968, see entire article (1996
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