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Human embryonic pluripotent germ cells

Patent 6090622 Issued on July 18, 2000. Estimated Expiration Date: Icon_subject March 31, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

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Inventors

Assignee

Application

No. 829372 filed on 03/31/1997

US Classes:

435/366, Human424/93.21, Eukaryotic cell435/325ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE

Examiners

Primary: Weber, Jon P.

Attorney, Agent or Firm

Foreign Patent References

  • 90/03432 A1 WO. 04/13/1990
  • 95-10599 WO. 04/13/1995
  • 97/20035 WO. 06/13/1997
  • 97/25413 WO. 07/13/1997
  • 97/25412 WO. 07/13/1997

International Classes

C12N 005/08
C12N 005/00

Abstract

Primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a 8-11 week LMP human embryo, are disclosed. The primordial germ cells are cultured in long term culture (more than 30 days) resulting in cells that resemble embryonic stem cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.

Other References

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