Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases
Patent 6090589 Issued on July 18, 2000. Estimated Expiration Date: 
July 18, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
July 18, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesAutocatalytic replication of recombinant RNA 4957858 Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA Nucleic acid sequence amplification methods Patent #: 5480784 Inventors
AssigneeApplicationNo. 480041 filed on 06/06/1995US Classes:435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/6, Involving nucleic acid435/91.21, Involving the making of multiple RNA copies536/22.1, N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.33, Primers536/25.4Separation or purification of polynucleotides or oligonucleotidesExaminersPrimary: Marschel, Ardin H.Assistant: Riley, Jezia Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C12P 019/34 C07H 019/00 C07H 021/04 AbstractThe present invention entails methods, and kits for carrying them out, based on the discovery that an RNA replicase, such as Qଲ replicase, has DNA-dependent RNA polymerase ("DDRP") activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2'-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase. The discovery of this DDRP activity provides methods of the invention for nucleic acid amplification wherein a nucleic acid, with a DNA segment with the sequence of an RNA that is autocatalytically replicatable by an RNA replicase, is provided as a substrate for the replicase. Assays of the invention include those wherein a nucleic acid analyte is hybridized with one or more nucleic acid probes, which include or are processed to generate a DNA segment which is amplifiable through production from the segment, catalyzed by the DDRP activity of an RNA replicase, of an autocatalytically replicatable RNA, which is autocatalytically replicated to provide an abundance of readily detectable reporter molecules.Other References
Field of SearchN-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.)Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Primers Separation or purification of polynucleotides or oligonucleotides Involving the making of multiple RNA copies Involving nucleic acid Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.) |
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