Creatine amidinohydrolase, production thereof and use thereof
Patent 6080553 Issued on June 27, 2000. Estimated Expiration Date: 
February 13, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
February 13, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3806420 3907644 Creatine amidinohydrolase from alkaligenes sp. ks-85 ferm bp-4487 Patent #: 5451520 InventorsAssigneeApplicationNo. 799897 filed on 02/13/1997US Classes:435/18, Involving hydrolase435/192, Acting on hydrogen peroxide as acceptor (1.11)435/227, Acting on carbon to nitrogen bond other than peptide bond (3.5)435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/252.33, Escherichia (e.g., E. coli, etc.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/829AlcaligenesExaminersPrimary: Slobodyansky, ElizabethAttorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/34C12N 009/78 C12N 001/20 C12N 001/00 Foreign Application Priority Data1996-02-13 JPAbstractA creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H2 O→sarcosine+ureaOptimum temperature: about 40-50° C.Optimum pH: pH about 8.0-9.0Heat stability: not more than about 50° C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mMMolecular weight: about 43,000 (SDS-PAGE)Isoelectric point: about 3.5,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.Field of SearchInvolving hydrolaseActing on carbon to nitrogen bond other than peptide bond (3.5) Bacteria or actinomycetales; media therefor VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) Alcaligenes Acting on hydrogen peroxide as acceptor (1.11) Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.) Escherichia (e.g., E. coli, etc.) |
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