In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants
Patent 6040188 Issued on March 21, 2000. Estimated Expiration Date: 
November 18, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 18, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod for solid phase membrane mimetics Immobilized artificial membranes Method of diagnosing irregularities in bile salt absorption via brush border membrane proteins Screening method for the identification of bioenhancers through the inhibition of P-glycoprotein transport in the gut of a mammal Method for detecting hydrocarbons in soil Patent #: 5756357 InventorApplicationNo. 196618 filed on 11/18/1998US Classes:436/71, LIPIDS, TRIGLYCERIDES, CHOLESTEROL, OR LIPOPROTEINS436/60, LUBRICANT, GREASE, MINERAL OIL, HYDROCARBON OIL PRODUCT, OR FATS OR LIPIDS FOR OXIDATION (E.G., BREAKDOWN PRODUCTS OR CONTAMINATION, ETC.)436/139, HYDROCARBON436/140AromaticExaminersPrimary: Warden, JillAssistant: Tran, Huan Attorney, Agent or FirmInternational ClassG01N 033/92AbstractThe present invention relates to measurements of contaminants in the soil and other organic or environmental materials, using a biologically relevant chemical analysis that will measure the amount of contaminants in a given sample that may be expected to be absorbed by a human being ingesting the contaminated soil. According to the present invention, environmental samples to be tested are added to a pre-prepared physiological composition of bile salts and lipids. They are thoroughly mixed and then the resulting mixture is separated e.g. by centrifugation. The supernatant is then analyzed for the presence of contaminants and these concentrations are compared to the level of contaminants in the untreated samples. It is important that the bile salts and lipids be thoroughly pre-mixed to form micelles.Other References
Field of SearchLUBRICANT, GREASE, MINERAL OIL, HYDROCARBON OIL PRODUCT, OR FATS OR LIPIDS FOR OXIDATION (E.G., BREAKDOWN PRODUCTS OR CONTAMINATION, ETC.)LIPIDS, TRIGLYCERIDES, CHOLESTEROL, OR LIPOPROTEINS Aromatic HYDROCARBON Digestion or removing interfering materials Liberation or purification of sample or separation of material from a sample (e.g., filtering, centrifuging, etc.) PRODUCT BLEND, E.G., COMPOSITION, ETC., OR BLENDING PROCESS PER SE Containing aromatic ring COMPOUND OR REACTION PRODUCT MIXTURE By addition of extraneous agent, e.g., solvent, etc. PURIFICATION, SEPARATION, OR RECOVERY |
| ||||||||||||||
