Packaging systems for human recombinant adenovirus to be used in gene therapy
Patent 6033908 Issued on March 7, 2000. Estimated Expiration Date: 
July 15, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
July 15, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethods and compositions for gene therapy for the treatment of defects in lipoprotein metabolism Adenovirus vector for gene therapy Adenovirus vectors for gene therapy Patent #: 5707618 InventorsApplicationNo. 892873 filed on 07/15/1997US Classes:435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE424/93.21, Eukaryotic cell435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/455, Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cell514/44, Polynucleotide (e.g., RNA, DNA, etc.)536/23.1DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)ExaminersPrimary: Campell, Bruce R.Assistant: Nguyen, Hiep T. Attorney, Agent or FirmForeign Patent References
International ClassC12N 015/00Foreign Application Priority Data1995-06-15 EPAbstractThe invention provides improved methods and products based on adenoviral materials which can advantageously be used in for instance gene therapy. In one aspect an adenoviral vector is provided which has no overlap with a suitable packaging cell line which is another aspect of invention. This combination excludes the possibility of homologous recombination, thereby excluding the possibility of the formation of replication competent adenovirus. In another aspect an adenovirus based helper construct which by its size is incapable of being encapsidated. This helper virus can be transferred into any suitable host cell making it a packaging cell. Further a number of useful mutations to adenoviral based materials and combinations of such mutations are disclosed, which all have in common the safety of the methods and the products, in particular avoiding the production of replication competent adenovirus and/or interference with the immune system. Further a method of intracellular amplification is provided.Other References
Field of SearchPolynucleotide (e.g., RNA, DNA, etc.)Eukaryotic cell VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE Recombinant DNA technique included in method of making a protein or polypeptide Introduction of a polynucleotide molecule into or rearrangement of nucleic acid within an animal cell DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) |
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