Fused gene and method of making and using same
Microbiological synthesis of beta endorphin
Method for microbial polypeptide expression
Microbial polypeptide expression vehicle
Polypeptide-toxin hybrid protein
Fusion peptides comprising an expressible bacterial gene and ଲ-endorphin
Thiolated polypeptide compound derived from a tetanus toxin fragment, the process for its obtention and its applications
Inactivating protein synthesis by incubating anti-Thy 1.1-ricin a chain monoclonal antibody hybrids with target protein cells
Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
Process for the specific cleavage of protein sequences from proteins
ApplicationNo. 479510 filed on 06/07/1995
US Classes:530/350, PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES530/351, Lymphokines, e.g., interferons, interlukins, etc.530/387.3, Chimeric, mutated, or recombined hybrid (e.g., bifunctional, bispecific, rodent-human chimeric, single chain, rFv, immunoglobulin fusion protein, etc.)530/388.1Monoclonal
ExaminersPrimary: Degen, Nancy
Assistant: Schwartzman, Robert A.
Attorney, Agent or Firm
Foreign Patent References
International ClassesC07K 014/00
AbstractDisclosed is a hybrid molecule comprising a first part, a second part, and a third part connected by covalent bonds,(a) wherein said first part comprises a portion of the binding domain of a cell-binding polypeptide ligand effective to cause said hybrid protein to bind to a cell of an animal;(b) wherein said second part comprises a portion of a translocation domain of naturally occurring protein which translocates said third part across the cytoplasmic membrane into the cytosol of the cell; and(c) wherein said third part comprises a chemical entity to be introduced into the cell, wherein each of said first part and said third part is non-native with respect to said naturally occurring protein, and further wherein said covalent bond connecting said second part and said third part is a cleavable bond, provided that when said second part comprises a portion of a translocation domain of Pseudomonas exotoxin, said third part is not a polypeptide.
Field of Search25 or more amino acid residues in defined sequence
PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES
Chemical modification or the reaction product thereof, e.g., covalent attachment or coupling, etc.
Chimeric, mutated, or recombined hybrid (e.g., bifunctional, bispecific, rodent-human chimeric, single chain, rFv, immunoglobulin fusion protein, etc.)
Lymphokines, e.g., interferons, interlukins, etc.