U.S. patents available from 1976 to present.
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Apparatus and method for detecting nucleic acid amplification products

Patent 6015674 Issued on January 18, 2000. Estimated Expiration Date: Icon_subject March 20, 2018. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Method and apparatus for background correction in photoluminescent analysis
Patent #: 4150295
Issued on: 04/17/1979
Inventor: Wieder

Indirect colorimetric detection of an analyte in a sample using ratio of light signals
Patent #: 4954435
Issued on: 09/04/1990
Inventor: Krauth

Tetrabenztriazaporphyrin reagents and kits containing the same
Patent #: 5135717
Issued on: 08/04/1992
Inventor: Renzoni, et al.

Method for detecting nucleic acid amplification using self-quenching fluorescence probe
Patent #: 5538848
Issued on: 07/23/1996
Inventor: Livak, et al.

Detection of nucleic acid amplification
Patent #: 5547861
Issued on: 08/20/1996
Inventor: Nadeau, et al.

Selective amplification system using Q-ଲ replicase
Patent #: 5556751
Issued on: 09/17/1996
Inventor: Stefano

Method for detection of nucleic acid using total internal reflectance
Patent #: 5585242
Issued on: 12/17/1996
Inventor: Bouma, et al.

Fluorerscence polarization detection of nucleic acid amplication Patent #: 5593867
Issued on: 01/14/1997
Inventor: Walker, et al.

Inventors

Assignee

Application

No. 046114 filed on 03/20/1998

US Classes:

435/6, Involving nucleic acid422/82.05, Measuring optical property by using ultraviolet, infrared, or visible light422/82.08, Fluorescence422/82.09, Absorbance or transmittance435/91.2Acellular exponential or geometric amplification (e.g., PCR, etc.)

Examiners

Primary: Horlick, Kenneth R.

Attorney, Agent or Firm

Foreign Patent References

  • 0 421 156 A2 EP 10/24/1991
  • 0 512 334 A2 EP 11/24/1992

International Classes

C12Q 001/68
C12P 019/34

Abstract

An apparatus is provided which includes a sample holder for holding a reaction chamber which includes an optical interface, a fiber optic cable for delivering an excitation beam to a sample housed within the reaction chamber and for receiving light emitted by the sample, and a lens co-axially disposed with the fiber optic cable and positioned outside the reaction chamber for focusing the excitation beam through the optical interface and within a volume of the sample and for collecting and transmitting to the fiber optic cable light emitted within the volume of the sample.

Other References

  • Lee, L. et al., "Allelic discrimination by nick-translation PCR with fluorogenic probes", Nucleic Acids Research, vol. 21, No. 16, pp. 3761-3766 (1993)
  • Higuchi, R. et al., "Simultaneous Amplification and Detection of Specific DNA Sequences", Bio-Technology, vol. 10, Apr. 1992, pp. 413-417
  • Gershoni, J. et al., "The Use of an Internal Standard for Semiquantitative Analysis of Low Temperature (77K) Fluorescence of Photosyntheic Cells", Analytical Biochemistry, vol. 104, pp. 315-320, (1980)
  • Krause, G. et al., "Characterization of Chlorophyll Fluorescence Quenching in Chloroplasts by Fluorescence Spectroscopy at 77K", Biochimica et Biophysica Acta., vol. 723, pp. 169-175 (1983
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