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Abstract

The present invention relates to an electrophoretic method to effect differential net migration, the extent of said migration being dependent on molecular size, of electrically charged macromolecules through a gel support in a single dimension, which method comprises subjecting electrically charged macromolecules applied to a gel support to an electric field oriented along a single axis within the gel for a time period sufficient to effect migration in the direction of the oriented field and form a separation pattern in order of the respective molecular weights of the macromolecules in a distance of about 0.5 to about 20 millimeters, said gel support comprising about 3 to about 40 percent polyacrylamide, said electric field being applied in an amount of about 5 to about 100 volts per millimeter of gel support along the axis length, and said gel support having a width perpendicular to said axis of about 0.1 to 1.5 millimeters.

Other References

• "Chemical Abstracts"vol. 111, No. 8, Aug. 21, 1998, Abstract No. 111:647545 Orban, Laszlo et al. "Sieving of ionic constituents across boundaries in gel eletrophoresis, " 10 (4) 254-259
• Orban, Laszlo et al. "Sieving of ionic constituents across boundaries in gel eletrophoresis," 10(4) 254-259
• B. Lambert et al. "Microelectrophoretic Technique for Fracionation of RNA" Nature, vol. 218 (Apr. 20, 1968) 292-293
• B. Lambert et al. "Extraction and Fractionation of Low Molecular Weight RNA on the Microscale "Nature vol. 220(Dec. 7, 1968) 1036-1037
• B. Lambert et al, "Agarose-Acrylamide Composite Gels for Microfractionation of RNA" Nature, vol. 220 (Dec. 7, 1968) 1037-1039
• Jorgenson & Lukacs, Capillary Zone Electrophoresis Science 222, pp. 266-272 (1983)
• Slater, Grant G., "Stable Pattern Formation and a Determination of Molecular Size by Pore-Limit Electrophoresis", Analytical Chemistry, vol. 41, No. 8, Jul. 1969, pp. 1039-1041
• Andrews, Anthony T., "Electrophoresis, theory, Techniques and Biochemical and Clinical Application", Second Edition, 1987
• Andrews, "Electrophoresis, Theory, Techniques and Biochemical and Clinical Applications", 2nd ed. pp. 7-8, 93-116, 153-164, 329-331, Oxford University Press, N.Y. no month available 1986
• Campbell et al., Anal. Biochem., 129:31-36 No Month Available (1983)
• Compton et al., BioTechniques, 6:432-440 No Month Available (1988)
• Drossman et al.,Anal. Chem., 62:900-903 No Month Available(1990)
• Guttman et al., Anal. Chem., 62:137-141 No Month Available (1990)
• Jeppesen, Anal. Biochem., 58: 195-207 No Month Available (1974)
• Luckey et al., Nucl. Acids Res., 18:4417-4421No Month Available (1990)
• Manwell, Biochem. J., 165:487-495 No Month Available (1977)
• Matioli et al., Science, 150: 1824-1826 No Month Available (1965)
• Neuhoff et al., Biochem. J., 117;623-631 (1970)
• Sambrook et al., "Molecular Cloning", 2nd ed., pp. 6.2-6.63, Cold Spring Harbor Laboratory Press, N.Y. *(1989