Use of nuclear magnetic resonance to design ligands to target biomolecules
Patent 5989827 Issued on November 23, 1999. Estimated Expiration Date: October 31, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay436/173, NUCLEAR MAGNETIC RESONANCE, ELECTRON SPIN RESONANCE OR OTHER SPIN EFFECTS OR MASS SPECTROMETRY436/501BIOSPECIFIC LIGAND BINDING ASSAY
The present invention provides a process of designing compounds which bind to a specific target molecule. The process includes the steps of a) identifying a first ligand to the target molecule using two-dimensional 15 N/1 H NMR correlation spectroscopy; b) identifying a second ligand to the target molecule using two-dimensional 15 N/1 H NMR correlation spectroscopy; c) forming a ternary complex by binding the first and second ligands to the target molecule; d) determining the three dimensional structure of the ternary complex and thus the spatial orientation of the first and second ligands on the target molecule; and e) linking the first and second ligands to form the drug, wherein the spatial orientation of step (d) is maintained.
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October 31, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
