U.S. patents available from 1976 to present.
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Basic protein composition, basic peptide composition and application thereof

Patent 5976597 Issued on November 2, 1999. Estimated Expiration Date: Icon_subject January 21, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Nutritionally improved foodstuffs
Patent #: 3970520
Issued on: 07/20/1976
Inventor: Feldman ,   et al.

Modified protein
Patent #: 4107334
Issued on: 08/15/1978
Inventor: Jolly

Process for extracting pure fractions of lactoperoxidase and lactoferrin from milk serum
Patent #: 5149647
Issued on: 09/22/1992
Inventor: Burling

Transparent acid drink containing acid-soluble casein phosphopeptide
Patent #: 5405756
Issued on: 04/11/1995
Inventor: Naito, et al.

Separation of lactoperoxidase, secretory component and lactoferrin from milk or whey with a cation exchange resin Patent #: 5516675
Issued on: 05/14/1996
Inventor: Uchida, et al.

Inventors

Assignee

Application

No. 785326 filed on 01/21/1997

US Classes:

426/491, Lacteal liquid, e.g., milk, cream, etc.426/271, INVOLVING ION EXCHANGE, SEQUESTERING OR CHELATING MATERIAL426/580, Basic ingredient lacteal derived other than butter substitute in emulsion form426/587, Liquid condensed or evaporated milk426/588, Powdered milk426/590, Beverage or beverage concentrate514/773, Peptide containing514/775Casein (milk protein) or derivative

Examiners

Primary: Eisenschenk, Frank C.
Assistant: Zeman, Mary K.

Attorney, Agent or Firm

Foreign Patent References

  • 0 556 083 A1 EP 08/11/1993
  • 0 573 668 A1 EP 12/11/1993
  • 0 704 218 A2 EP 04/11/1996
  • 2303457 JP. 12/11/1990
  • 04183371 JP. 06/11/1992
  • 07033668 JP. 02/11/1995
  • 08165249 JP. 06/11/1996
  • WO93/13676 WO 07/11/1993

International Classes

A23C 001/00
A23C 007/04
A23C 009/146
A23C 009/14

Foreign Application Priority Data

1996-01-23 JP

Abstract

The present invention provides a basic protein and a basic peptide composition which can be administered orally and has the ability to stimulate osteoblast proliferation, strengthen bone and inhibit bone resorption. Said basic protein has the following properties: (1) a molecular weight distribution of is 2,000-24,000; (2) an isoelectric point distribution of is 7.5-11; and (3) it is comprised of more than 10% of basic amino acids. Said composition can be obtained by the following steps: After milk, or raw material derived from milk, is loaded onto a cation exchange resin column, a fraction is obtained by eluting with an eluent of 0.1-1.0M salt concentration, precipitate removed by (i) heating to over 75° C., (ii) by adding alcohol so that the final concentration thereof is 10-50% or (iii) by adding salts so that the final concentration thereof will be more than 0.2M; and the supernatant thereof containing the basic protein fraction recovered.

Other References

  • K. Kussendrager, "Lactoferrin and Lactoperoxydase, Bioactive Milk Proteins" IFI NR.6, pp. 17-21 (1993)
  • S. Rudloff et al., "Calcium Retention from Milk-Based Infant Formulas, Whey-Hydrolysate Formula, and Human Milk in Weanling Rhesus Monkeys" AJDC 144:360-363 (1990)
  • Francis et al. 1995 J Dairy Sci 78 pages 1209-1218, May 1995
  • European Patent Office Search Report, App. No. EP 97 10 0945, Aug. 20, 1998, 3 pgs
  • Yukihiro Takada et al., "Whey Protein Stimulates the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells," Biomedical and Biophysical Research Communications, 223, pp. 445-449 (1996)
  • Database WPI, Section Ch, Week 9635, "Bone Strengthing Composition for Increasing Calcium Absorption, Osteoporosis--Contains Basic Protein Fraction and Calcium Derived from Milk, Especially Useful for Children," Derwent Publications Ltd., London, GB, Class B04, AN 96-350156, XP-00207391
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