Patent 5939303 Issued on August 17, 1999. Estimated Expiration Date: November 6, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
Novel phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponema and Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNA's encoding the novel phytases and host cells transformed with DNA's encoding the novel phytases are also provided. The novel phytases are useful in a wide range of applications involving the dephosphorylation of phytate, including, among other things, use in animal feed supplements.
Other References
Ausubel, F.A. Brent, R., Kingston, R.E., Moore, D.D., Sneidman, J.G. Smith, J.A. and Struhl, K. (eds.) 1990. Current Protocols in Molecular Biology. Green Publishing and Wiley-Interscience, New York
Jurgen Brosius, Mary Erfle and Storella, John 1985. Spacing of the -10 and -35 regions in the tac Promoter. J. Biol. Chem. 260:3539-3541
Bryant, M.P. and Burkey, L.A. 1953. Cultural Methods and Some Characteristics of Some of the Numerous Groups of Bacteria in the Bovine Rumen. J. Dairy Sci. 36:205-217
Cheng, E.W., Hall, Glen andBurroughs, Wise 1955. A Method for the Study of Cellulose Digeston by Washed Suspensions of Rumen Microorganisms. J. Dairy Sci. 38:1255-1230
Cheng, K.-J. and Costerton, J.W. 1973. Localization of Alkaline Phosphatase in Three Gram-Negative Rumen Bacteria. J. Bacteriol. 116:424-440
Ellis, S.B., Brust, P.F., Koutz, P.J., Waters, A.F., Harpold, M.M. and Gingeras, T.R. 1985. Isolation of Alcohol Oxidase and Two Other Methanol Regulated Genes from the Yeast Pichia pastoris. Mol. Cell. Biol. 5:1111-1121
Fiske, Cyrus H. and Subbarow, Yellapragada 1925. The Colorimetric Determination of phosphorus. J. Biol. Chem. 66:376-400
Gelvin, Stanton B., Schilperoort, R.A. and Verma, D.P.S. (eds.) 1993. Plant Molecular Biology Manual. Kluwer Academic Publishers, Boston, MA
Howson, S.J. and Davs, R.P. 1983. Production of Phytase-hydrolysing Enzyme by Some Fungi. Enzyme Microb. Technol. 5:377-382
Hu, Y.J., Smith, D.C., Cheng, K.-J. and Forsberg, Cecil W. 1991. Cloning of a Xylanase Gene from Fibrobacter succinogenes 135 and its Expression in Escherichia coli. Can.J. Microbiol. 37:554-561
Laemmli, U.K. 1970. Cleavage of the Structural Proteins During Assembly of the Head of Bacteriophage T4. Nature 227:680-685
Priefer, U., Simon, R. and Puhler, A. 1984. Cloning with Cosmids. In: Puhler, A. And Timmis, K.N. (eds). Advanced Molecular Genetics. Springer-Verlag, New York 190-201 pp
Raun, Arthur, Cheng, E. And Buroughs, W. 1956. Phytate phosphorus Hydrolysis and Availability to Rumen Microorganisms. Agric. Food Chem. 4:869-871
Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning. A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY
Scott, H.W. and Dehority, B.A. 1965. Vitamin Requirements of Several Cellulolytic Bacteria. J. Bacteriol. 89:1169-1175
Shieh, T.R. and Ware, J.H. 1968. Survey of Microorganisms for the Production of Extracellular Phytase, Appl. Microbiol. 16:1348-1351
van Hartingsveldt, Wim, van Zeijl, C.M.J. Harteveld, G.M., Gouka, R.J., Suykerbuyk, M.E.G. Luiten, R.G.M., van Paridon, P.A., Selten, G.C.M., Veenstra, A.E., van Gorcum, R.F.M. and van den Hondel, C.A.M.J. 1993. Cloning, Characterization and Overexpression of the Phytase-Encoding Gene (phyA) of Aspergillus niger. Gene 127:87-94
van Rooijen, Gijs J.H. and Moloney, M.M. 1994. Plant Seed Oil-Bodies as Carriers for Foreign Proteins. Bio/Technology 13:72-77
von Heijne, Gunnar 1986. A New Method for Predicting Signal Sequence Cleavage Sites. Nucleic Acids Res. 14:4683-4690
Won, Sui-Lam 1989. Development of an Inducible and Enhancible Expression and Secretion System in Bacillus subtilis. Gene 83:215-223
Wiryawan, K.G. et al. Autralian Journal of Agricultural Research 46(8): 1555-1568 (abstract only cited), 1995
Punj, M.L. et al. (1969), "Utilization of Phytin Phosphorus by Rumen Microorganisms," Ind. Vet. J. 46(10):881-886, at p. 885
Dayhoff, M.O., Schwartz, R.M. and Orcutt, B.C. 1978. A Module of Evoluntionary Change in Proteins. In: Atlas of Protein Sequence and Structure. vol. 5. Supplement 3,22:345-35