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Phytases of ruminal microorganisms

Patent 5939303 Issued on August 17, 1999. Estimated Expiration Date: Icon_subject November 6, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

3297548

Cloning and expression of phytase from aspergillus Patent #: 5436156
Issued on: 07/25/1995
Inventor: Van Gorcom, et al.

Inventors

Assignee

Application

No. 744779 filed on 11/06/1996

US Classes:

435/196, Acting on ester bond (3.1)424/94.6, Hydrolases (3. ) (e.g., urease, lipase, asparaginase, muramidase, etc.)426/61, DORMANT FERMENT CONTAINING PRODUCT, OR LIVE MICROORGANISM CONTAINING PRODUCT OR ONGOING FERMENTING PRODUCT, PROCESS OF PREPARATION OR TREATMENT THEREOF426/635, Animal food435/183, ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES435/195Hydrolase (3. )

Examiners

Primary: Mosher, Mary E.

Attorney, Agent or Firm

Foreign Patent References

  • 0420358A1 EP. 04/13/1991
  • 0449375A2 EP. 10/13/1991
  • 684313 EP 11/13/1995
  • 699762 EP 03/13/1996
  • WO 93/16175 WO. 08/13/1993
  • WO 94/03072 WO. 02/13/1994

International Classes

C12N 009/16
A61K 038/48
A23K 001/00

Abstract

Novel phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponema and Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNA's encoding the novel phytases and host cells transformed with DNA's encoding the novel phytases are also provided. The novel phytases are useful in a wide range of applications involving the dephosphorylation of phytate, including, among other things, use in animal feed supplements.

Other References

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