AbstractThe invention is directed to attenuated canine parvovirus (CPV) strains which may be used as a veterinary vaccine against CPV disease. The invention is further directed to a virus stock generated from a genomic DNA clone of the attenuated CPV strain which is used as a veterinary vaccine and is able to confer protective immunity to dogs against challenge with virulent CPV. Methods are given for the production of an attenuated CPV virus from a cloned CPV genome which may be used as a veterinary vaccine. Other References- Parrish et al., Rapid Antigen-Type Replacement and DNA Sequence Evolution of Canine Parvovirus, J. Virol., vol. 65, No. 12, pp. 6544-6552, see p. 6545, left column , p. 6544, column 2, second full paragraph), Dec. 1991
- Parrish, Emergence, Natural History, and Variation of Canine, Mink, and Feline Parvoviruses, Adv. Virus Res. 38:403-450, see pp. 419-423, 1990
- Gruenberg et al., 1993, "Preparation and analysis of a molecular clone of an attenuated strain of canine parvovirus" Abstract, 5th Parvovirus Workshop, Nov. 10-14, 1993, Cyrstal River, Florida
- Lopez de Turison et al., 1992, "Recombinant vaccine for canine parvovirus in dogs", J Virol 66(5):2748-2753
- Saliki et al., 1992, "Canine parvovirus empty capsids produced by expression in a baculovirus vector: Use in analysis of viral properties and immunization of dogs", J Gen Virol 73:369-374
- Parrish et al., 1991 "Mapping specific functions in the capsid structure of canine parvovirus and feline panleukipenia virus using infectious plasmid clones", Virology 183:195-205
- Parrish et al., 1991, "Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus", J Virol 65(12):6544-6552
- Parrish, 1990, "Emergence, natural history, and variation of canine, mink, and feline parvovirus", Adv Virus Res 38:403-450
- Ausubel et al., 1989, Current Potocols in Molecular Biology, Greene Publshing Associates and Wiley Interscience, Chapter 8
- Chang et al., 1988, "Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties", J Virol 66(12):6858-6867
- Mazzara et al., 1987, "Successful vaccination of dogs with empty capsids derived from canine parvovirus-bovine papillomavirus chimeric plasmids", Vaccines 87, Cold Spring Harbor Laboratory Press, pp. 419-424
- Botstein and Shortle, 1985, "Strategies and applications of in vitro mutagenesis", Science 229:1193-1201
- Parrish et al., 1984, "Characterization of antigenic variation among mink enteritis virus isolates", Am J Vet Res 45:2591-2599
- Zoller and Smith, 1984, "Oligonucleotide-directed mutagenesis: A simple method using two oligonucliotide primers and a single stranded DNA template", DNA 3(6):479-488
- Carmichael et al., 1983, "A modified live canine parvovirus vaccine II. Immune Response", Cornell Vet 73:13-29
- Carmichael et al. 19881, "A modified live canine parvovirus strain with novel plaque characteristics I. Viral attenuation and dog response", Cornell Vet 71:408-427
- Carmichael et al., 1980 "Hemagglutination by canine parvovirus: Serologic studies and diagnostic applications", Am J Vet Res 41(5):784-791
- Appel et al., 1979, "Isolation and immunisation studies of a canine parvo-like virus from dogs with haemorrhagic enteritis", Vet Record 105:156-159
- Bovarnick et al., 1950, "The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae", J Bact 59:509-522
- Gruenberg et al., 1993, Poster Panels from an oral presentation given at the Fifth Parvovirus Workshop, Crystal City, Florida, Nov. 10-14, 1993
- Churchill, 1987, "Preliminary Devleopment of a Live Attenuated Canine Parvovirus Vaccine from an isloate of British origin", The Veterinary Record 120:334-33
| InventorsAssigneeApplication No. 647655 filed on 05/15/1996 US Classes:424/204.1, Virus or component thereof424/186.1, Disclosed amino acid sequence derived from virus435/235.1, VIRUS OR BACTERIOPHAGE, EXCEPT FOR VIRAL VECTOR OR BACTERIOPHAGE VECTOR; COMPOSITION THEREOF; PREPARATION OR PURIFICATION THEREOF; PRODUCTION OF VIRAL SUBUNITS; MEDIA FOR PROPAGATING435/236, Inactivation or attenuation; producing viral subunits536/23.72Viral proteinField of Search424/186.1, Disclosed amino acid sequence derived from virus424/204.1, Virus or component thereof435/235.1, VIRUS OR BACTERIOPHAGE, EXCEPT FOR VIRAL VECTOR OR BACTERIOPHAGE VECTOR; COMPOSITION THEREOF; PREPARATION OR PURIFICATION THEREOF; PRODUCTION OF VIRAL SUBUNITS; MEDIA FOR PROPAGATING435/236, Inactivation or attenuation; producing viral subunits536/23.72Viral proteinExaminers Primary: Achutamurthy, Ponnathapu Assistant: Park, Hankyel T.Attorney, Agent or FirmUS Patent References4193990, Heterotypic canine parvovirus vaccine Issued on: 03/18/1980 Inventor: Appel , et al.4193991, Canine parvovirus vaccine Issued on: 03/18/1980 Inventor: Appel , et al.4303645, Modified living canine parvovirus vaccine Issued on: 12/01/1981 Inventor: Carmichael , et al.4810494, Canine parvovirus vaccines Issued on: 03/07/1989 Inventor: Welsh4971793Subunit canine parvovirus vaccine Issued on: 11/20/1990 Inventor: Wood, et al.Foreign Patent References- 554 414 A2 EP. 11/20/1993
International Classes A61K 039/12 C12N 007/00 C12N 007/04 C07H 021/04
|