3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
February 16, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesCytidine nucleoside compound Nucleosides and process Oligonucleotide derivatives Process for the simultaneous synthesis of several oligonucleotides on a solid phase Enzyme-free diagnostic binding reagents Chemical capping by phosphitylation during oligonucleotide synthesis Oligonucleotide derivatives and production thereof Automatable process for sequencing nucleotide Poly-labelled oligonucleotide derivative Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis InventorsAssigneeApplicationNo. 486535 filed on 06/07/1995US Classes:536/26.26, Triphosphates (in same chain)536/26.6, Labelled (e.g., tagged with radioactive tracer, fluorescent marker, intercalator, etc.)536/26.7The N-hetero ring is part of a bicyclic ring systemExaminersPrimary: Wilson, James O.Attorney, Agent or FirmForeign Patent References
International ClassC07H 019/00AbstractA method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.Other References
Field of SearchInvolving nucleic acidSynthesis of polynucleotides or oligonucleotides Deprotection step Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.) Pentavalent phosphorus compound utilized Trivalent phosphorus compound utilized Triphosphates (in same chain) Labelled (e.g., tagged with radioactive tracer, fluorescent marker, intercalator, etc.) The N-hetero ring is part of a bicyclic ring system |
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