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Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems

Patent 5869004 Issued on February 9, 1999. Estimated Expiration Date: Icon_subject June 9, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

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Inventors

Assignee

Application

No. 871338 filed on 06/09/1997

US Classes:

422/100, Pipette or other volumetric fluid transfer means73/863, SAMPLER, SAMPLE HANDLING, ETC.204/600, Electrophoretic or electro-osmotic apparatus422/58, In holder or container having special form422/68.1, Means for analyzing liquid or solid sample436/52, With a continuously flowing sample or carrier stream436/179, Dilution436/180Volumetric liquid transfer

Examiners

Primary: Warden, Robert J.
Assistant: Moazzam, Fariborz

Attorney, Agent or Firm

Foreign Patent References

  • WO 94/05414 WO 03/13/1994
  • WO 96/04547 WO 02/13/1996
  • WO 97/02357 WO 01/13/1997

International Classes

B01L 003/00
B01L 011/00

Abstract

The present invention is generally directed to methods, apparatus and systems for use in performing in situ dilution or concentration of a particular subject material in a microfluidic device or system. These methods and apparatus may generally be integrated with other microfluidic operations and/or systems, to perfom a number of different manipulations, wherein dilution or concentration, carried out within the context of the microfluidic device or system, is just one part.

Other References

  • Bao, J. et al., "Ultramicro enzyme assays in capillary electrophoretic system," J. Chrom. 608:217-224 (1992)
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  • Ramsey, J.M. et al., "Microfabricated chemical measurement systems," Nature Medicine 1(10):1093-1096 (1995)
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  • Seiler, K. et al., "Planar Glass Chips for Capillary Electrophoresis: Repetitive Sample Injection, Quantitation, and Separation Efficiency," Anal. Chem. 65:1481-1488 (1993)
  • Woolley, A.T. et al., "Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips," Pro. Natl. Acad. Sci. U.S.A. 91:11348-11352 (1994
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