Flow cell SELEX
January 31, 2017. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesHigh-affinity RNA ligands of basic fibroblast growth factor Methods for identifying nucleic acid ligands of human neutrophil elastase Ligands of thrombin Surface plasmon resonance sensor unit and its use in biosensor systems Nucleic acid ligands to HIV-RT and HIV-1 rev Method for identification of high affinity DNA ligands of HIV-1 reverse transcriptase Ligands of HIV-1 tat protein DNA ligands of thrombin Systematic evolution of ligands by exponential enrichment: Solution SELEX High-affinity nucleic acid ligands that discriminate between theophylline and caffeine InventorsApplicationNo. 792075 filed on 01/31/1997US Classes:435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)536/25.4Separation or purification of polynucleotides or oligonucleotidesExaminersPrimary: Zitomer, Stephanie W.Attorney, Agent or FirmForeign Patent References
International ClassesC12P 019/34C12Q 001/68 C07H 021/02 G01N 021/01 AbstractDescribed herein are methods for improved partitioning between high and low affinity nucleic acid ligands identified through the SELEX method, termed Flow Cell SELEX. The Flow Cell SELEX method achieves partitioning between high and low affinity nucleic acid ligands using surface plasmon resonance technology. The method of the present invention presents a new and powerful approach to select nucleic acid ligands by providing a partitioning method which 1) enables a significant increase in the efficiency of partitioning from traditional partitioning methods used in SELEX, 2) allows for real time monitoring of the partitioning of the high affinity ligands from the low affinity ligands 3) allows for the ability to select for a nucleic acid ligand having specific kinetic properties, 4) does not rely on radiolabeling or other means of tagging for detection, and 5) allows for use of smaller amounts of target than in traditional methods of SELEX.Other References
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