Erythropoietin isoforms

Patent 5856298 Issued on January 5, 1999. Estimated Expiration Date:

January 5, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Process for the production of human erythropoietin
Patent #: 4377513
Issued on: 03/22/1983
Inventor: Sugimoto , et al.

Erythropoietin purification
Patent #: 4667016
Issued on: 05/19/1987
Inventor: Lai , et al.

DNA sequences encoding erythropoietin
Patent #: 4703008
Issued on: 10/27/1987
Inventor: Lin

PG gene and its use in plants
Patent #: 4801540
Issued on: 01/31/1989
Inventor: Hiatt , et al.

Synthetic gene for acyl carrier protein
Patent #: 4888282
Issued on: 12/19/1989
Inventor: Beremand, et al.

Plastin isoforms and their use
Patent #: 5002870
Issued on: 03/26/1991
Inventor: Leavitt, et al.

Method of purifying protein Patent #: 5451662
Issued on: 09/19/1995
Inventor: Naveh, et al.

Inventor

Strickland, Thomas Wayne

Application

No. 334882 filed on 11/03/1994

US Classes:

514/8, Glycoprotein (carbohydrate containing)204/459, Isoelectric focusing (i.e., using pH variation)435/69.4, Hormones and fragments thereof530/395, Glycoprotein, e.g., mucins proteoglycans, etc.530/397, Glycoprotein hormones530/412, Separation or purification530/416Ion exchange

Examiners

Primary: Walsh, Stephen

Attorney, Agent or Firm

Winter; Robert B., Odre; Steven M., Levy; Ron K.

Foreign Patent References

267 678 EP. 05/13/1988
0409113 EP. 01/13/1991

International Classes

A61K 038/18
C07K 014/505
C07K 001/18
C07K 001/28

Abstract

Erythropoietin isoforms having a specific number of sialic acids per erythropoietin molecule are disclosed. Also disclosed are mixtures of such isoforms, pharmaceutical compositions containing such isoforms or mixtures thereof and methods of obtaining the erythropoietin isoforms.

Other References

Fuhr et al., Biochemical and Biophysical Research Communications 98: 930-935, Feb. 27,1981
Viau et al., Biochemical and Biophysical Research Communications 117: 324-331, Nov. 30, 1983
Havel et al., Proc. Natl. Acad. Sci. 77: 4349-4353, Jul. 1980
Welch et al., Journal of Biological Chemistry 259: 4501-4513, Apr. 1984
Napier et al "Isoelectric Focussing of Human Urinary Erythropoietiu" IRCS Med. SCI4:437 (1976)
Andreux et al "Etude de l'erythropoietiue. . . " Annales Pharma. Francaises 31(1):29-36, (1973)
Fuhr et al., Biochem. & Biophys. Res. Comm.98: 930-935, Feb. 27, 1981
Viau et al., Biochem. & Biophys. Res. Comm. 117: 324-331, Nov/ 30, 1983
Havel et al., PNAS 77: 4349-4353, Jul. 1980
Krystal et al., Blood 67: 71-79, Jan. 1986
Welch et al., J. Biol. Chem. 259: 4501-4513, Apr. 1984
Dordal et al., Endocrinology 116: 2293-2299, 1985
Sasaki et al., J. Biol. Chem. 262:12059-12076, Sep. 5, 1987
Copsey et al., Genetically Eng. Human Therap. Drugs, pp. 25-26, 257, 1988
Dube et al., J. Biol. Chem. 263:17516-17521, 25 Nov. 1988
Goldwasser, "Erythropoietiu & red cell differentiation", in Control of Cellular Division and Devel: Part A, pp. 487-494, 1981
Lukowsky et al, "Studies on the role of Sialic Acid in The hysical and Biological Properties of Erythropoietin", Can. J. Biochem. 50:909-917, 1972
Ashwell et al. Methods Enzymol. 50 , 287-288 (1978)
Bradford Anal. Biochem. 72 , 248-254 (1976)
Burnette et al. Anal. Biochem. 112 , 195-203 (1981)
Cotes et al. Nature 191 , 1065-1067 (Sep. 1961)
Davis et al. Biochemistry 26 , 2633-2638 (1987)
Egrie et al. Immunobiology 172l ,213-224 (1986)
Elliott et al. Blood 74 ,Supp. 1, A 1228 (1989)
Elliott et al. Gene 79 , 167-180 (1989)
Fukuda et al. Blood 73 , 84-89 (Jan. 1989)
Goldwasser et al. J. Biol. Chem. 249 , 4202-4206 (Jul. 1974)
Holmes et al. Anal. Biochem. 117 , 193 (1981)
Iscove et al. J.Cell Physiol. 83 , 309-320 (1974)
Kunkel et al. Methods Enzymol. 154 , 367-382 (1987)
Lai et al. J. Biol. Chem. 261 , 3116-3121 (Mar. 1986)
Law et al. Proc. Natl. Acad. Sci. USA 83 , 6920-6924 (Sep. 1986)
Lee et al. J. Biol. Chem. 264 , 13848-13855 (Aug. 1989)
Lin et al. Proc. Natl. Acad. Sci. USA 82 , 7580-7584 (Nov. 1985)
Messing Methods Enzymol. 101 , 20-78 (1983)
Miyake et al. J. Biol. Chem., 252 , 5558-5564 (Aug. 1977)
Morrell et al. J. Biol Chem 243, 155-59 (Jan. 1968)
Mutsaers et al. Eur. J. Biochem. 156 , 651-654 (1986)
Phelps et al. Biochem. Biophys. Acta. 791 , 226-238 (1984)
Shelton et al Biochem. Med. 12 , 45-54 (1975)
Takeuchi et al. J. Biol. Chem. 263 , 3657-3663 (Mar. 1988)
Takeuchi et al. J. Chromatogr. 400 , 207-213 (1987)
Yanisch-Perron et al. Gene 33 , 103-119 (1985)
Dorado et al. Biochem. Medicine 6 , 238-245 (1972)
Napier IRCS Med. Sci. Biochem. 4 , 437 (1976