Methods for hybridization analysis utilizing electrically controlled hybridization
Patent 5849486 Issued on December 15, 1998. Estimated Expiration Date: September 27, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/6, Involving nucleic acid257/E21.43, Recessing gate by adding semiconductor material at source (S) or drain (D) location, e.g., transist or with elevated single crystal S and D (EPO)257/E21.435, Lateral single gate single channel silicon transistor with both lightly doped source and drain extensions and source and drain self-aligned to sides of gate, e.g., LDD MOSFET, DDD MOSFET (EPO)257/E29.267, With nonplanar structure (e.g., gate or source or drain being nonplanar) (EPO)436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/25.3Synthesis of polynucleotides or oligonucleotides
A system for performing molecular biological diagnosis, analysis and multistep and multiplex reactions utilizes a selfaddressable, selfassembling microelectronic system for actively carrying out controlled reactions in microscopic formats. Preferably, a fluidic system flow a sample across an active area of the biochip, increasing diagnostic efficiency. Preferably, the fluidic system includes aflow cell having a window. Pulsed activation of the electrodes of the biochip are advantageously employed with the fluidic system, permitting more complete sampling of the materials within the biological sample. An improved detection system utilizes a preferably coaxially oriented excitation fiber, such as a fiber optic, disposed within a light guide, such as a liquid light guide. In this way, small geometry systems may be fluorescently imaged. A highly automated DNA diagnostic system results. Perturbation of the fluorescence signal during electronic denaturation is detailed and analyzed for analytical and diagnostic purposes. Such fluorescence perturbation information is combined with other information to provide improved analysis. DNA fingerprinting uses hybridizing DNA fragments of a given length and a capture sequence at a test site and then determining the level of reverse bias necessary to affect the hybridization, such as to dehybridize, and determine the length of the DNA.
Other References
Abrams et al. (1990) Genomics, vol. 7, pp. 463-475
Brown et al, "Electrochemically Induced Adsorption of Radio-Labelled DNA on Gold and HOPG Substrates for STM Investigations", Ultramicroscopy 38 (1991) 253-264
Washizu, et al., "Electrostatic Manipulation of DNA in Microfabricated Structures", IEEE Transactions on Industrial Applications, vol. 26, No. 6, Nov./Dec., 1990, pp. 1165-1172
Palecek, "New Trends in Electrochemical Analysis of Nucleic Acids", Bioelectrochemistry and Bioenergetics, 20 (1988), pp. 179-194
Connor et al., "Detection of sickle cell ଲg -globin allele by hybridization with synthetic oligonucleotides," Proc. Natl. Acad. Sci. USA, 80:278-282 (1983)
Drmanac et al., "Sequencing of Megabase Plus DNA by Hybridization: Theory of the Method," Genomics, 4:114-128 (1989)
Drmanac et al., "DNA Sequence Determination by Hybridization: A Strategy for Efficient Large-Scale Sequencing," Science, 260:1649-1652 (1993)
Fodor et al., "Multiplexed biochemical assays with biological chips," Nature, 364:555-556 (1993)
Fodor et al., "Light-Directd, Spatially Addressable Parallel Chemical Synthesis," Science, 251:767-773 (1991)
Horejsi, "Some Theoretical Aspects of Affinity Electrophoresis," Journal of Chromatography, 178:1-13 (1979)
Horejsi et al., "Determination of Dissociation Constants of Lectin Sugar Complexes by Means of Affinity Electrophoresis", Biochimica et biophysica acta, 499:200-300 (1977)
Ranki et al., "Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples," Gene, 21:77-85 (1983)
Saiki, "Amplification of Genomic DNA," PCR Protocols: A Guide to Methods and Applications, (Academic Press, Inc. 1990) pp. 13-20
Southern et al., "Analyzing and Comparing Nucleic Acid Sequences by Hybridization to Arrays of Okigonucleotides: Evaluation Using Experimental Models," Genomics, 13:1008-1017 (1992)
Strezoska et al., "DNA sequencing by hybridization: 100 bases read by a non-gel-based method," Proc. Natl. Acad. Sci. USA, 88:10089-10093 (1991)
Wallace et al., "Hybridization of synthetic oligodcoxyribonucleotides to .PHI. x 174 DNA: the effect of single base pair mismatch," Nucleic Acid Res., 6:3543-3557 (1979)
Washizu, "Electrostatic manipulation of biological objects," Journal of Electrostatics, 25:109-123 (1990)
Washizu and Kurosawa, "Electrostatic Manipulation of DNA in Microfabricated Structures," IEEE Transactions on Industry Applications, 26:1165-1172 (1990)
Anand and Southern, "Pulsed field gel electrophoresis," Gel Electrophoresis of Nucleic Acids--A Practical Approach, 2d edition, eds. D. Rickwood and B.D. Hames, (New York:IRL Press 1990) pp. 101-123
Anderson and Young, "Quantitative Filter Hybridisation," Nucleic Acid Hybridization--A Practical Approach, eds. B.D. Hames and S.J. Higgins (Washington DC:IRL Press 1985) pp. 73-111
Bains, "Setting a Sequence to Sequence a Sequence," Bio/Technology, 10:757-758 (1992)
Beattie et al., "Genosensor Technology," The 1992 San Diego Conference: Genetic Recognition, pp. 1-5, (Nov., 1992)
Beltz et al., "Isolation of Multigene Families and Determination of Homologies by Filter Hybridization Methods," Methods in Enzymology, 100:266-285 (1983
September 27, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
